Anti-HA tag antibody [16B12] (DyLight® 650) (ab117515)

Overview

  • Product name
    Anti-HA tag antibody [16B12] (DyLight® 650)
    See all HA tag primary antibodies
  • Description
    Mouse monoclonal [16B12] to HA tag (DyLight® 650)
  • Conjugation
    DyLight® 650. Ex: 654nm, Em: 673nm
  • Specificity
    ab117515 recognizes the Influenza hemagglutinin epitope (YPYDVPDYA).
  • Tested applications
    Suitable for: ICC/IF, Flow Cytmore details
  • Immunogen

    Synthetic peptide

    YPYDVPDYA

    corresponding to HA tag.

  • General notes

    Stable for 1yr.

    Conjugated to Dylight® 650 (ratio 1.3:): Visible absorption maxima 652 nm; Emission maximum 672 nm.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 8.20
    Constituent: 99% Tris buffered saline
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    16B12
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/5000.
Flow Cyt Use at an assay dependent concentration.

Target

  • Relevance
    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Anti-HA tag antibody [16B12] (DyLight® 650) images

  • ab117515 staining 293T cells expressing a HA-tagged protein (intracellular) by Flow Cytometry. Cells were permeabilized and fixed. The sample was incubated with the primary antibody (1/200) for 30 minutes at 4°C.

    Gating Strategy: Gated on FSC-SSC to exclude debris/dead cells.

    Grey - unstained cells.

    Red - Wild type 293T, stained.

    Blue - 293T expressing HA-tagged protein, stained.

    See Abreview

References for Anti-HA tag antibody [16B12] (DyLight® 650) (ab117515)

This product has been referenced in:
  • Greenwood EJ  et al. Temporal proteomic analysis of HIV infection reveals remodelling of the host phosphoproteome by lentiviral Vif variants. Elife 5:N/A (2016). Read more (PubMed: 27690223) »

See 1 Publication for this product

Product Wall

Application
Flow Cytometry
Fixation
BD Fix/Perm
Permeabilization
Yes - BD Perm/Wash
Sample
Human Cell (293T)
Specification
293T
Gating Strategy
Gated on FSC-SSC to exclude debris/dead cells
Preparation
Cell harvesting/tissue preparation method: WT and cells expressing an HA-tagged protein (intracellular)
Sample buffer: BD fix/perm and perm/wash
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Verified customer

Submitted Mar 11 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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