Anti-HA tag antibody [16B12] (Phycoerythrin) (ab72479)

Overview

  • Product name
    Anti-HA tag antibody [16B12] (Phycoerythrin)
    See all HA tag primary antibodies
  • Description
    Mouse monoclonal [16B12] to HA tag (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity
    ab72479 recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Immunogen

    Raised against the twelve amino acid peptide CYPYDVPDYASL.

  • Epitope
    ab72479 recognizes the influenza hemagglutinin epitope (YPYDVPDYA).
  • General notes

    Excitation max. lambda: 565>498 nm Emission max. lambda: 578 nm.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 2mM Sodium Azide
    Constituents: 0.1% BSA, 150mM Sodium chloride, 100mM Sodium phosphate
  • Concentration information loading...
  • Purity
    Size exclusion
  • Purification notes
    Size exclusion chromatography as well as proprietary methods for purifying the final materials were used.
  • Clonality
    Monoclonal
  • Clone number
    16B12
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Images

  • Flow cytometry analysis of a recombinant (N-terminus HA-tag) transmembrane cell surface receptor in CHO cells using ab72479 (blue). Black - Mouse IgG1 Phycoerythrin isotype control.

References

This product has been referenced in:
  • Yugawa T  et al. Noncanonical NOTCH signaling limits self-renewal of human epithelial and induced pluripotent stem cells through ROCK activation. Mol Cell Biol 33:4434-47 (2013). Read more (PubMed: 24019071) »

See 1 Publication for this product

Customer reviews and Q&As

ab72479:
Like most phycobiliprotein conjugates, the ratio of the fluor to the antibody (F/P) can vary slightly from lot to lot due to the nature of the conjugation chemistry and the methods used to characterize the final product, but it is typicall...

Read More

ab72479 recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus. You should be able to use it for intracellular staining or extracellular staining

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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