Anti-HA tag antibody [16B12] (Phycoerythrin) (ab72479)


  • Product nameAnti-HA tag antibody [16B12] (Phycoerythrin)
    See all HA tag primary antibodies
  • Description
    Mouse monoclonal [16B12] to HA tag (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Specificityab72479 recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Immunogen

    Raised against the twelve amino acid peptide CYPYDVPDYASL.

  • Epitopeab72479 recognizes the influenza hemagglutinin epitope (YPYDVPDYA).
  • General notes

    Excitation max. lambda: 565>498 nm Emission max. lambda: 578 nm.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 2mM Sodium Azide
    Constituents: 0.1% BSA, 150mM Sodium chloride, 100mM Sodium phosphate
  • Concentration information loading...
  • PuritySize exclusion
  • Purification notesSize exclusion chromatography as well as proprietary methods for purifying the final materials were used.
  • ClonalityMonoclonal
  • Clone number16B12
  • IsotypeIgG1
  • Research areas


Our Abpromise guarantee covers the use of ab72479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Anti-HA tag antibody [16B12] (Phycoerythrin) images

  • Flow cytometry analysis of a recombinant (N-terminus HA-tag) transmembrane cell surface receptor in CHO cells using ab72479 (blue). Black - Mouse IgG1 Phycoerythrin isotype control.

References for Anti-HA tag antibody [16B12] (Phycoerythrin) (ab72479)

This product has been referenced in:
  • Yugawa T  et al. Noncanonical NOTCH signaling limits self-renewal of human epithelial and induced pluripotent stem cells through ROCK activation. Mol Cell Biol 33:4434-47 (2013). Read more (PubMed: 24019071) »

See 1 Publication for this product

Product Wall

Like most phycobiliprotein conjugates, the ratio of the fluor to the antibody (F/P) can vary slightly from lot to lot due to the nature of the conjugation chemistry and the methods used to characterize the final product, but it is typicall...

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ab72479 recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus. You should be able to use it for intracellular staining or extracellular staining.

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