Anti-HA tag antibody [2-2.2.14] (HRP) (ab173826)

Overview

  • Product name
    Anti-HA tag antibody [2-2.2.14] (HRP)
    See all HA tag primary antibodies
  • Description
    Mouse monoclonal [2-2.2.14] to HA tag (HRP)
  • Conjugation
    HRP
  • Tested applications
    Suitable for: WBmore details
  • Immunogen

    Synthetic peptide corresponding to HA tag aa 1-9 conjugated to Ovalbumin.
    Sequence:

    YPYDVPDYA

  • Positive control
    • E. coli lysate containing a multi-epitope tagged protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    2-2.2.14
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab173826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 1 kDa.

Target

  • Relevance
    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Images

  • All lanes : Anti-HA tag antibody [2-2.2.14] (HRP) (ab173826) at 1/1000 dilution

    Lane 1 : E. coli lysate containing a multi-epitope tagged protein at 1 µg
    Lane 2 : E. coli lysate containing a multi-epitope tagged protein at 0.5 µg
    Lane 3 : E. coli lysate containing a multi-epitope tagged protein at 0.25 µg
    Lane 4 : E. coli lysate containing a multi-epitope tagged protein at 0.125 µg
    Lane 5 : E. coli lysate containing a multi-epitope tagged protein at 0.0500000007451 µg

    Developed using the ECL technique

    Predicted band size : 1 kDa

    Note: the predicted MW indicated is for the HA tag.

References

ab173826 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (S. cerevisiae cell lysate)
Specification
S. cerevisiae cell lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

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Verified customer

Submitted Mar 04 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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