Overview

  • Product nameAnti-HA tag antibody [2C16] (Biotin)
    See all HA tag primary antibodies
  • Description
    Mouse monoclonal [2C16] to HA tag (Biotin)
  • ConjugationBiotin
  • SpecificityThis antibody detects HA tags fused to the C terminus or N terminus of targeted proteins in transfected mammalian cells.
  • Tested applicationsSuitable for: WB, IPmore details
  • Immunogen

    Synthetic peptide - (YPYDVPDYA), derived from the human influenza hemaglutinin protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, 1.0% BSA, PBS, pH 7.2
  • Concentration information loading...
  • Purification notesDialyzed.
  • ClonalityMonoclonal
  • Clone number2C16
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at a concentration of 2 - 10 µg/ml.
    WB: Use at a concentration of 2 - 10 µg/ml.
    Immunostaining techniques of 2 - 10 µg/ml

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
    • Alternative names
      • HA epitope tag antibody
      • HA1 antibody
      • HA2 antibody
      • hemagglutinin antibody
      • Hemagglutinin HA1 chain antibody
      • Hemagglutinin HA2 chain antibody
      see all

    References for Anti-HA tag antibody [2C16] (Biotin) (ab27987)

    This product has been referenced in:
    • Volná P  et al. Flow cytometric analysis of DNA binding and cleavage by cell surface-displayed homing endonucleases. Nucleic Acids Res 35:2748-58 (2007). Flow Cyt . Read more (PubMed: 17426121) »

    See 1 Publication for this product

    Product Wall

    This antibody has actually been used in the following publication:

    Volná P et al. Flow cytometric analysis of DNA binding and cleavage by cell surface-displayed homing endonucleases. Nucleic Acids Res 35:2748-58 (2007).

    If you...

    Read More

    Thank you for your reply.
    If the antibody ab27987 fails to work in either an IP or western blot and we cannot help you identify the cause of this and solve this issue for you, then yes you would be eligible for a refund or replacement.
    You ca...

    Read More

    I am sorry that you are disappointed with one of our products. As you have already opened and used the product, we are unable to accept it as a return.
    Under our Abpromise we guarantee our products to work in the applications and species listed on...

    Read More

    Thank you for contacting us.
    We unfortunately haven't done any test on the degree of Biotinylation for this antibody. The antibody is however guaranteed to be used in applications as listed on the datasheet. I am afraid we will not be able to prov...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"