Overview

  • Product nameAnti-HA tag antibody [2C16] (FITC)
    See all HA tag primary antibodies
  • Description
    Mouse monoclonal [2C16] to HA tag (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityThe antibody detects HA-tag fused to the C-terminus or N-terminus of targeted proteins in transfected cells.
  • Tested applicationsSuitable for: Flow Cyt, ICC/IFmore details

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide
  • Concentration information loading...
  • ClonalityMonoclonal
  • Clone number2C16
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
ICC/IF
  • Application notesFlow Cyt: Use at an assay dependent dilution (PMID 14647427).
    IF: Use at a concentration of 2 - 10 µg/ml.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
    • Alternative names
      • HA epitope tag antibody
      • HA1 antibody
      • HA2 antibody
      • hemagglutinin antibody
      • Hemagglutinin HA1 chain antibody
      • Hemagglutinin HA2 chain antibody
      see all

    References for Anti-HA tag antibody [2C16] (FITC) (ab1789)

    This product has been referenced in:
    • Caruso N  et al. Deregulation of the protocadherin gene FAT1 alters muscle shapes: implications for the pathogenesis of facioscapulohumeral dystrophy. PLoS Genet 9:e1003550 (2013). ICC/IF . Read more (PubMed: 23785297) »
    • Yamashiro H  et al. Stimulation of human butyrophilin 3 molecules results in negative regulation of cellular immunity. J Leukoc Biol 88:757-67 (2010). Flow Cyt . Read more (PubMed: 20610803) »

    See all 5 Publications for this product

    Product Wall

    When we know what the recommended dilutions are for an antibody we will state them on the datasheet, otherwise the dilutions are assay dependent. When they are assay dependent we advise customers to follow Abcam's general protocols which will advise on...

    Read More

    The HA epitope sequence: YPYDVPDYA.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"