The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use at an assay dependent dilution (PMID 14647427).
IF: Use at a concentration of 2 - 10 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
HA epitope tag antibody
Hemagglutinin HA1 chain antibody
Hemagglutinin HA2 chain antibody
References for Anti-HA tag antibody [2C16] (FITC) (ab1789)
This product has been referenced in:
Caruso N et al. Deregulation of the protocadherin gene FAT1 alters muscle shapes: implications for the pathogenesis of facioscapulohumeral dystrophy. PLoS Genet9:e1003550 (2013).
Read more (PubMed: 23785297) »
Yamashiro H et al. Stimulation of human butyrophilin 3 molecules results in negative regulation of cellular immunity. J Leukoc Biol88:757-67 (2010).
Read more (PubMed: 20610803) »