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Our Abpromise guarantee covers the use of ab1424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/1000. PubMed: 17167031|
|ChIP||Use a concentration of 3 µg/ml. Use 3 µg per 25 µg chromatin (see figure legend).|
|ICC||Use a concentration of 10 µg/ml.|
|IP||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 1 µg/ml.|
This picture shows baby hamster kidney cell expressing HA-tag CFTR protein. This type of cells were grown on coverslip and immunofluorescence was performed. Dilution of 1:200 was used and incubated for 2hrs with antibody.
Recombinant HA-tagged Rab6 was immunoprecipitated from 1 mg transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 2 μL ab1424.
Lane 1: Rab6-HA transfected HEK-293T whole cell extract (Input)
Lane 2: ab1424 IP in Rab6-HA transfected HEK-293T whole cell extract.
Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS. Chromatin was prepared according to the Abcam X-ChIP protocol. Oocytes were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 3µg of ab1424 (anti-HA, light blue) and 3µg of ab18337 (anti-BORIS, dark blue), and 20µl of Protein A/G sepharose beads. A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).
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