Overview

  • Product name
    Anti-HA tag antibody [4C12]
    See all HA tag primary antibodies
  • Description
    Mouse monoclonal [4C12] to HA tag
  • Specificity
    The antibody detects HA-tag fused to the C-terminus or N-terminus of targeted proteins in transfected cells. The antibody works in ChIP but less well than ab9110 (see ChIP data).
  • Tested applications
    Suitable for: ELISA, ChIP, ICC, IP, WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide
  • Concentration information loading...
  • Clonality
    Monoclonal
  • Clone number
    4C12
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000. PubMed: 17167031
ChIP Use a concentration of 3 µg/ml. Use 3 µg per 25 µg chromatin (see figure legend).
ICC Use a concentration of 10 µg/ml.
IP Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml.

Target

  • Relevance
    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Images

  • Anti-HA tag antibody [4C12] (ab1424) at 1/1000 dilution + HEK293T whole cell lysate over-expressing HA-tagged Rab6 at 20 µg

    Secondary
    HRP-conjugated goat anti-mouse polyclonal IgG at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 5 seconds

    This image is courtesy of an anonymous Abreview

    Blocked with 5% milk for 30 min at 25°C

    See Abreview

  • This picture shows baby hamster kidney cell expressing HA-tag CFTR protein. This type of cells were grown on coverslip and immunofluorescence was performed. Dilution of 1:200 was used and incubated for 2hrs with antibody.

  • Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS. Chromatin was prepared according to the Abcam X-ChIP protocol. Oocytes were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 3µg of ab1424 (anti-HA, light blue)  and 3µg of ab18337 (anti-BORIS, dark blue), and 20µl of Protein A/G sepharose beads. A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).

References

This product has been referenced in:
  • Zhou C  et al. Novel nesprin-1 mutations associated with dilated cardiomyopathy cause nuclear envelope disruption and defects in myogenesis. Hum Mol Genet 26:2258-2276 (2017). Read more (PubMed: 28398466) »
  • Hilmi K  et al. CTCF facilitates DNA double-strand break repair by enhancing homologous recombination repair. Sci Adv 3:e1601898 (2017). Read more (PubMed: 28560323) »

See all 21 Publications for this product

Customer reviews and Q&As

Thank you for your reply.

You can find out more about the 100% Abreview Testing Discount Program below:

http://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount



For all...

Read More

Thank you for contacting Abcam. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which gua...

Read More

Thank you for contacting us. Both antibodies have not yet been tested in any IHC application (neither IHC-P or IHC-Fr). We do have a testing discount program that you can use in that case. Here is some information about how the testing d...

Read More

Thank you for your enquiry. The immunogen was YPYDVPDYAC. Please let me know if you have any more questions.

Thank you for your enquiry. Jen is out of the office, so I am answering her correspondence. I understand that you used a third antibody successfully in this protocol, but each antibody is different and sometimes must be optimized to work well. W...

Read More

Thank you for your phone call and I'm sorry to hear that you are experiencing difficulty with this antibody. We have not received any other complaints regarding this batch and so it's a possibility that this latest vial which you received went off at s...

Read More

Thank you for providing me with details regarding your protocol and also for the image you submitted. We have had some good feedback regarding this antibody in Western blotting, and customers have reported seeing a clean signal using ab1424 at a diluti...

Read More

Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab1424. Could we get a detailed protocol from you, please? We have some general questions, the answers to which will enable us to investigate this matter as quic...

Read More

Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with this antibody. Without knowing more details regarding your protocol, I would first suggest decreasing the concentrations of the primary and secondary antibodies ...

Read More

Thank you for your customer's details regarding their protocol. At this point I would recommend decreasing the concentration of the primary antibody and to also run a secondary antibody control (no primary) to ensure that this cross-reactivity is not c...

Read More

1-10 of 13 Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up