Anti-HA tag antibody [4C12] - ChIP Grade (ab1424)

Overview

  • Product nameAnti-HA tag antibody [4C12] - ChIP GradeSee all HA tag primary antibodies ...
  • Description
    Mouse monoclonal [4C12] to HA tag - ChIP Grade
  • SpecificityThe antibody detects HA-tag fused to the C-terminus or N-terminus of targeted proteins in transfected cells. The antibody works in ChIP but less well than ab9110 (see ChIP data).
  • Tested applicationsELISA, ChIP, ICC, IP, WB more details

Properties

Applications

Our Abpromise guarantee covers the use of ab1424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA 1/1000. PubMed: 17167031
ChIP Use a concentration of 3 µg/ml. Use 3 µg per 25 µg chromatin (see figure legend).
ICC Use a concentration of 10 µg/ml.
IP Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml.

Target

  • RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Anti-HA tag antibody [4C12] - ChIP Grade images

  • This picture shows baby hamster kidney cell expressing HA-tag CFTR protein. This type of cells were grown on coverslip and immunoflourescence was performed. Dilution of 1:200 was used and incubated for 2hrs with antibody.
  • Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS. Chromatin was prepared according to the Abcam X-ChIP protocol. Oocytes were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 3µg of ab1424 (anti-HA, light blue)  and 3µg of ab18337 (anti-BORIS, dark blue), and 20µl of Protein A/G sepharose beads. A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).

  • Anti-HA tag antibody [4C12] - ChIP Grade (ab1424) at 1/1000 dilution + HEK293T whole cell lysate over-expressing HA-tagged Rab6 at 20 µg

    Secondary
    HRP-conjugated goat anti-rabbit polyclonal IgG at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 5 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-HA tag antibody [4C12] - ChIP Grade (ab1424)

This product has been referenced in:
  • Tessarz P  et al. Glutamine methylation in histone H2A is an RNA-polymerase-I-dedicated modification. Nature 505:564-8 (2014). Read more (PubMed: 24352239) »
  • Narayanan MS & Rudenko G TDP1 is an HMG chromatin protein facilitating RNA polymerase I transcription in African trypanosomes. Nucleic Acids Res : (2013). Read more (PubMed: 23361461) »

See all 12 Publications for this product

Product Wall

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (Retro virus transduction for protein X)
Total protein in input 500 µg
Specification Retro virus transduction for protein X
Immuno-precipitation step Protein A/G
Username

Abcam user community

Verified customer

Submitted Feb 26 2013

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HEK 293T cells)
Total protein in input 1000 µg
Specification HEK 293T cells
Immuno-precipitation step Protein A
Username

Abcam user community

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Submitted Jan 16 2013

Application Western blot
Sample Human Cell lysate - whole cell (HEK293T)
Loading amount 20 µg
Specification HEK293T
Gel Running Conditions Reduced Denaturing (12% Tris-glycine gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Sep 17 2012

Thank you for your reply.

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Application Western blot
Sample Human Cell lysate - whole cell (human nueroblastoma cells)
Loading amount 25 µg
Specification human nueroblastoma cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Submitted Jan 28 2011

Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (HEK293)
Total protein in input 10 µg
Specification HEK293
Immuno-precipitation step Protein G
Username

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Submitted Jan 18 2008

Application Western blot
Sample Mouse Cell lysate - whole cell (HEK293)
Loading amount 10 µg
Specification HEK293
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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Submitted Jan 18 2008

Application Western blot
Sample Human Cell lysate - whole cell (HEK293T cell line)
Loading amount 30 µg
Specification HEK293T cell line
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
Username

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Submitted Dec 28 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"