Synthetic peptide corresponding to the HA-tag sequence.
This antibody gave a positive signal against an E. coli lysate that expresses a 41kDa recombinant protein comprised of 11 commonly used epitope tags (T7, HSV, C-Myc, VSV-g, Glu-Glu, V5, E-tag, FLAG, S-tag, HA and 6xHis). This lysate is available as ab5395.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
HA epitope tag antibody
Hemagglutinin HA1 chain antibody
Hemagglutinin HA2 chain antibody
Anti-HA tag antibody images
Western blot - Anti-HA tag antibody (ab107245)
Anti-HA tag antibody (ab107245) at 1 µg/ml + E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 10 µg
Secondary Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) at 1/3000 dilution Developed using the ECL technique