Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab13834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500. Can be blocked with HA tag peptide.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
    • Alternative names
      • HA epitope tag antibody
      • HA1 antibody
      • HA2 antibody
      • hemagglutinin antibody
      • Hemagglutinin HA1 chain antibody
      • Hemagglutinin HA2 chain antibody
      see all

    Anti-HA tag antibody images

    • All lanes : Anti-HA tag antibody (ab13834) at 1/500 dilution

      Lane 1 : HEK293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896)
      Lane 2 : HEK293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896) with HA tag peptide (ab13835) at 1 µg/ml

      Lysates/proteins at 10 µg per lane.

      Secondary
      Alexa Fluor 680 Goat-anti-Rabbit IgG at 1/5000 dilution

      Performed under reducing conditions.

      Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    References for Anti-HA tag antibody (ab13834)

    This product has been referenced in:
    • Huang Y  et al. UXT-V1 protects cells against TNF-induced apoptosis through modulating complex II formation. Mol Biol Cell 22:1389-97 (2011). WB ; Human . Read more (PubMed: 21307340) »
    • Alhamidi M  et al. Fukutin-Related Protein Resides in the Golgi Cisternae of Skeletal Muscle Fibres and Forms Disulfide-Linked Homodimers via an N-Terminal Interaction. PLoS One 6:e22968 (2011). WB . Read more (PubMed: 21886772) »

    See all 3 Publications for this product

    Product Wall

    Application IHC - Wholemount
    Sample Mouse Tissue (Brain)
    Specification Brain
    Username

    Prof. Vidita Vaidya

    Verified customer

    Submitted Jul 23 2014

    Thank you for your enquiry, I am pleased to help.

    From what I understand here, the customer would liketo detect the HA tag on their recombinant protein expressed in cell culture. This is Immunocytochemistry (ICC). I have reviewed the selecti...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa cells)
    Loading amount 40 µg
    Specification HeLa cells
    Gel Running Conditions Reduced Denaturing (10% Bis-Tris)
    Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
    Username

    Dr. Svetlana Khoronenkova

    Verified customer

    Submitted Aug 17 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Human glioma cells)
    Loading amount 250000 cells
    Specification Human glioma cells
    Treatment Transfected with lipofectamine
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 04 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"