Overview

  • Product name
  • Description
    Rabbit polyclonal to HA tag
  • Tested applications
    Suitable for: WB, ChIP, ICC/IFmore details
  • Species reactivity
    Not appplicable.
  • Immunogen

    Synthetic peptide:

    CYPYDVPDYAYPYDVPDYA

    encoding HA tag conjugated to KLH

  • Positive control
    • HA-tagged recombinant protein bacterial lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 50 kDa.
ChIP Use at an assay dependent concentration.
ICC/IF 1/10 - 1/50.

Target

  • Relevance
    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Anti-HA tag antibody images

  • All lanes : Anti-HA tag antibody (ab71113)

    Lane 1 : HA-tagged recombinant protein bacterial lysate
    Lane 2 : HA-tagged recombinant protein bacterial lysate

    Lysates/proteins at 12.5 µg per lane.


    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescent staining of MRC-5 fibroblasts transiently transfected with either wild-type (wt) or a GTPase-deficient dynamin-2 mutant (K44A) with a hemagglutinin (HA) tag using ab71113.

  • ChIP was performed with 35S:HATCL1 plants using anti-HA antibodies. Rabbit preimmune serum was used as a mock control. Primer sets specific for the first intron or the 3-UTR region of GL1 were used in PCR reactions. ACTIN2 was used as a control.

References for Anti-HA tag antibody (ab71113)

This product has been referenced in:
  • Duval N  et al. Msx1 and Msx2 act as essential activators of Atoh1 expression in the murine spinal cord. Development 141:1726-36 (2014). Read more (PubMed: 24715462) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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