Overview

  • Product name
  • Description
    Chicken polyclonal to HA tag
  • Specificity
    ELISA: this antibody diluted at 1:20,000 gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti peptide purification. HRP conjugated goat anti chicken IgG (IgY) was used and TMB was the substrate.
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Immunogen

    YPYDVPDYA (influenza hemagglutinin-HA (epitope) conjugated to KLH.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    PBS, pH 7.2, sodium azide, 0.1%
  • Concentration information loading...
  • Purity
    IgY fraction
  • Purification notes
    Antibodies were immunoaffinity purified using the peptide immobilized on a solid support.
  • Clonality
    Polyclonal
  • Isotype
    unknown
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. PubMed: 20943657
WB 1/1000.
ELISA Use at an assay dependent dilution.

Target

  • Relevance
    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Images

  • ab9111 staining HA tag (red) in 293T cells overexpressing KHAmCt-980 protein by Immunocytochemistry/ Immunofluorescence. Nucleoli stained with DAPI. Green is for specific speckle marker.

References

This product has been referenced in:
  • Berck ME  et al. The wiring diagram of a glomerular olfactory system. Elife 5:N/A (2016). Read more (PubMed: 27177418) »
  • Voelkel-Meiman K  et al. Separable Crossover-Promoting and Crossover-Constraining Aspects of Zip1 Activity during Budding Yeast Meiosis. PLoS Genet 11:e1005335 (2015). Bacteria . Read more (PubMed: 26114667) »

See all 6 Publications for this product

Customer reviews and Q&As

Unfortunately our expertise is in the field of antibody production and epitope design. We work closely with a range of collaborators to produce fusion or recombinant proteins and therefore are unable to offer the information that you require. We wis...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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