Overview

  • Product nameAnti-HA tag antibody
    See all HA tag primary antibodies
  • Description
    Chicken polyclonal to HA tag
  • SpecificityELISA: this antibody diluted at 1:20,000 gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti peptide purification. HRP conjugated goat anti chicken IgG (IgY) was used and TMB was the substrate.
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Immunogen

    YPYDVPDYA

    (influenza hemagglutinin-HA (epitope) conjugated to KLH.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPBS, pH 7.2, sodium azide, 0.1%
  • Concentration information loading...
  • PurityIgY fraction
  • Purification notesAntibodies were immunoaffinity purified using the peptide immobilized on a solid support.
  • ClonalityPolyclonal
  • Isotypeunknown
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. PubMed: 20943657
WB 1/1000.
ELISA Use at an assay dependent dilution.

Target

  • RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Anti-HA tag antibody images

  • ab9111 staining HA tag (red) in 293T cells overexpressing KHAmCt-980 protein by Immunocytochemistry/ Immunofluorescence. Nucleoli stained with DAPI. Green is for specific speckle marker.

References for Anti-HA tag antibody (ab9111)

This product has been referenced in:
  • Voelkel-Meiman K  et al. Separable Crossover-Promoting and Crossover-Constraining Aspects of Zip1 Activity during Budding Yeast Meiosis. PLoS Genet 11:e1005335 (2015). Bacteria . Read more (PubMed: 26114667) »
  • Liaunardy-Jopeace A  et al. The COP II adaptor protein TMED7 is required to initiate and mediate the delivery of TLR4 to the plasma membrane. Sci Signal 7:ra70 (2014). Read more (PubMed: 25074978) »

See all 4 Publications for this product

Product Wall

Unfortunately our expertise is in the field of antibody production and epitope design. We work closely with a range of collaborators to produce fusion or recombinant proteins and therefore are unable to offer the information that you require. We wis...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"