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Synthetic peptide corresponding to HA tag conjugated to Keyhole Limpet Haemocyanin (KLH).
Our Abpromise guarantee covers the use of ab9134 in the following tested applications.
|ICC/IF||Use at an assay dependent dilution.|
|IHC-Fr||Use at an assay dependent dilution. PubMed: 20551914|
|ELISA||1/100 - 1/500.|
|WB||1/1000 - 1/30000.|
|IP||Use at an assay dependent dilution.|
|ICC||1/200 - 1/2000.|
This image is courtesy of an abreview submitted by Dr Weiguo Zhang.
Immunofluorescence analysis of HeLa R1-11 cells transfected with wtFLAGhPCFTMyc-His10 or FLAGP425RMyc-His10 and wthPCFTHA or wthPCFTHA alone. HA tag was stained with ab9134.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and incubated with primary antibody. An Alexa Fluor® 568-conjugated anti-goat IgG was used as the secondary antibody.