• Product nameAnti-HA tag antibody (Agarose)
    See all HA tag primary antibodies
  • Description
    Goat polyclonal to HA tag (Agarose)
  • ConjugationAgarose
  • Tested applicationsSuitable for: IPmore details
  • Immunogen

    Full length native protein


    (HA) (purified): conjugated to KLH.

  • General notes

    Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.



Our Abpromise guarantee covers the use of ab1233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution. Use at a concentration of 20 - 40 µl of gel slurry per 0.1 - 1 mg of protein lysate or extract.


  • RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Anti-HA tag antibody (Agarose) images

  • ab1233 used in Immunoprecipitation. For further details please see reference.

References for Anti-HA tag antibody (Agarose) (ab1233)

This product has been referenced in:
  • Slagter-Jäger JG  et al. Evaluation of RNA Amplification Methods to Improve DC Immunotherapy Antigen Presentation and Immune Response. Mol Ther Nucleic Acids 2:e91 (2013). IP . Read more (PubMed: 23653155) »
  • Bullen HE  et al. A novel family of Apicomplexan glideosome-associated proteins with an inner membrane-anchoring role. J Biol Chem 284:25353-63 (2009). IP . Read more (PubMed: 19561073) »

See all 2 Publications for this product

Product Wall

Thank you for your inquiry.

Pre-clearing the lysates is always up to the end-user to determine because background levels will vary. Since both of these antibodies are conjugated to agarose, if you want to pre-clear the lysates you could...

Read More

The source of the antiboy informed me that the agarose beads we use are from Sterogene. The bead size listed is: 60-160µm. More information can be found from their website: http://www.sterogene.com/uniflow4.html

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HEK 293T cells)
Total protein in input 25 µg
Specification HEK 293T cells
Treatment Transfected with HA-tagged p300
Immuno-precipitation step Other - anti-HA agarose

Dr. Jonathan Rud

Verified customer

Submitted Jan 11 2010

Thank you for your enquiry. The volume for this product is 400 ul of 50% slurry containing 200 ul gel. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.