Overview

  • Product nameAnti-HA tag antibody - ChIP Grade
    See all HA tag primary antibodies
  • Description
    Rabbit polyclonal to HA tag - ChIP Grade
  • SpecificityELISA: The anti HA diluted 1:70.000 gave an O.D.=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification. HRP conjugated Goat anti rabbit IgG was used and TMB was the substrate.
  • Tested applicationsSuitable for: ChIP/Chip, IP, ELISA, WB, ICC/IF, ICC, Flow Cyt, ChIPmore details
  • Immunogen

    YPYDVPDYA

    (influenza hemagglutinin-HA-epitope) conjugated to KLH.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP/Chip Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ELISA 1/200 - 1/500.
WB 1/4000 - 1/10000.
ICC/IF Use a concentration of 1 - 4 µg/ml.
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ChIP Use 3 µg for 25 µg of chromatin.

Target

  • RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Anti-HA tag antibody - ChIP Grade images

  • All lanes : Anti-HA tag antibody - ChIP Grade (ab9110) at 1/4000 dilution

    Lane 1 : 15ug untransfected wcl lysate
    Lane 2 : 293FT cells transfected with 15kDa HA tagged Vpr (an HIV1 accessory protein)

    Secondary
    HRP conjugated Goat anti-Rabbit
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 5 seconds
    This image is courtesy of an Abreview

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  • ab9110 was diluted to 4 µg/mg lysate and incubated with a nuclear lysate of HEK293T cells transiently expressing HA-tagged protein and a Protein A matrix for 2 hours a 23°C to achieve immunoprecipitation.  1000 µg of lysate was present in the input. 

    A HRP-conjugated anti-rabbit HA monoclonal antibody diluted 1/1000 was used for the Western Blot step.

    See Abreview

  • This image was kindly supplied as part of the review submitted by Kasper Fugger. Immunofluorescence staining of U-2 cells expressing HA-tagged protein with ab9110.
  • Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS. Chromatin was prepared according to the Abcam X-ChIP protocol. Oocytes were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 20µl of Protein A/G sepharose beads, and 3µg of ab9110 (anti-HA, light blue) or, 3µg of ab18337 (anti-Boris, dark blue). A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).

  • ab 9110 at a 1/200 dilution staining the mouse olineu cell line (oligodendrocyte precursor cell) by immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected using a Cy5 conjugated goat anti-rabbit antibody.

    This image is courtesy of an Abreview submitted by Katarina Trajkovic on 15 March 2006

    See Abreview

References for Anti-HA tag antibody - ChIP Grade (ab9110)

This product has been referenced in:
  • Liu Z  et al. Mark4 promotes oxidative stress and inflammation via binding to PPAR? and activating NF-?B pathway in mice adipocytes. Sci Rep 6:21382 (2016). WB, IP . Read more (PubMed: 26888669) »
  • Kim DI  et al. An improved smaller biotin ligase for BioID proximity labeling. Mol Biol Cell 27:1188-96 (2016). ICC/IF . Read more (PubMed: 26912792) »

See all 323 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Embryonic kidney fibroblast cells)
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Loading amount 1e+006 cells
Specification Embryonic kidney fibroblast cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
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Submitted Apr 01 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Cell (spinal cord)
Permeabilization Yes - PBS/gelatin/triton/serum
Specification spinal cord
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative Paraformaldehyde
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Submitted Jul 14 2015

Application Western blot
Sample Chinese Hamster Cell lysate - whole cell (CHO cell)
Gel Running Conditions Reduced Denaturing
Loading amount 30 µg
Specification CHO cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Jun 23 2015

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (Transfected ES cells)
Specification Transfected ES cells
Treatment 1uM all-trans retinoic acid for 24hrs
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Feb 27 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 18°C
Sample Human Cell (293T)
Specification 293T
Permeabilization Yes - 0.5% Triton X100
Fixative Paraformaldehyde
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Submitted Aug 15 2014

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (4-12 Bis tris)
Sample Human Cell lysate - whole cell (293T)
Specification 293T
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Aug 15 2014

Application Immunoprecipitation
Immuno-precipitation step Protein G
Sample Human Cell lysate - whole cell (HeLa)
Specification HeLa
Treatment transfected with HA-tagged gene
Total protein in input 10 µg
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Submitted May 02 2014

Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Human Cell lysate - nuclear (Prostate cancer cell lines)
Specification Prostate cancer cell lines
Treatment 4 h DHT (or ETOH)
Total protein in input 5e+006 cells
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Laura Cato

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Submitted Apr 28 2014

Application Immunoprecipitation
Immuno-precipitation step A second antibody
Sample Mouse Cell lysate - whole cell (Embryonic stem cell)
Specification Embryonic stem cell
Total protein in input 500 µg
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Mr. Dan Stummer

Verified customer

Submitted Apr 03 2014

Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (HeLa)
Specification HeLa
Treatment transfected with HA-tagged gene
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
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Submitted Mar 29 2014

1-10 of 55 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"