Western blot abreview for Anti-HA tag antibody - ChIP Grade

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293FT)
Loading amount
15 µg
Specification
293FT
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Dilution
1/4000
Incubation time
18 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
5 second(s)
Bands
Specific: 15 kDa
Positive control
293FT transfected with a 15kDa HA tagged protein
Negative control
Untransfected 293FT wcl

Additional data

Additional Notes
Lane 1 is untransfected wcl lysates (15micrograms) Lane 2 is 293FT cells transfected with 15kDa HA tagged Vpr an HIV-1 accessory protein.
Username

Abcam user community

Verified customer

Submitted May 12 2006

Sign up