Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 0.2% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesResearchers should optimise dilutions for applications.
  • Target

    • RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
    • Alternative names
      • HA epitope tag antibody
      • HA1 antibody
      • HA2 antibody
      • hemagglutinin antibody
      • Hemagglutinin HA1 chain antibody
      • Hemagglutinin HA2 chain antibody
      see all

    Anti-HA tag antibody (FITC) images

    • ab1208 staining Ha tag in human breast cancer cells (HA tagged expression plasmid) by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.2% Triton X-100 in PBS and then blocked using 1% BSA and 3% FBS in PBS for 30 minutes at room temperature. Samples were then incubated with primary antibody at 1/50 for 1 hour.

      See Abreview

    References for Anti-HA tag antibody (FITC) (ab1208)

    ab1208 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Monkey Cell (cos7)
    Specification cos7
    Permeabilization No
    Fixative Formaldehyde
    Username

    Ms. YA ZHOU

    Verified customer

    Submitted Dec 13 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Breast cancer cells (HA tagged expression plasmid)
    Specification Breast cancer cells (HA tagged expression plasmid
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.2% Triton in PBS
    Blocking step 1% BSA and 3% FBS in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
    Username

    Dr. Manikandan Periyasamy

    Verified customer

    Submitted Sep 23 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"