Overview

  • Product nameAnti-HA tag antibody [HA-7]
    See all HA tag primary antibodies
  • Description
    Mouse monoclonal [HA-7] to HA tag
  • Tested applicationsSuitable for: ELISA, ICC/IF, IP, ICC, WBmore details
  • Immunogen

    Synthetic peptide:

    YPYDVPDYA

    conjugated to KLH, corresponding to amino acids 98-106 of Influenza A Virus Hemagglutinin HA tag

  • Positive control
    • Whole extracts of transfected 293T (human embryonal kidney) cells, expressing HA-tagged fusion protein.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR275412, GR177296. Lot numbers other than GR275412 and GR177296 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.097% Sodium azide
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberHA-7
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Use at an assay dependent concentration.

ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
WB 1/10000. Predicted molecular weight: 63 kDa.

Target

  • RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Anti-HA tag antibody [HA-7] images

  • ab49969 Immunoprecipitating HA tag in HA tagged protein transfected 293T whole cell lysate. 25µg of cell lysate was incubated with primary antibody (1/2500 in lysis buffer) and matrix (Protein A/G) for 6 hours at 4°C. For western blotting anti-mouse HRP (ab6789) (1/10000) was used to confirm successful immunoprecipation.

    75kD is specific band for HA tagged protein. 120kD, 55kD, 35kD is non specific band for protein A/G bead.

    See Abreview

References for Anti-HA tag antibody [HA-7] (ab49969)

This product has been referenced in:
  • Guo H  et al. The aspirin-induced long non-coding RNA OLA1P2 blocks phosphorylated STAT3 homodimer formation. Genome Biol 17:24 (2016). WB ; Human . Read more (PubMed: 26898989) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Mouse Cell (MEL (erythroluekemia cell))
Specification MEL (erythroluekemia cell)
Permeabilization No
Fixative Paraformaldehyde
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Abcam user community

Verified customer

Submitted Jul 10 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Sample Mouse Cell lysate - whole cell (MEL (erythroluekemia cell))
Specification MEL (erythroluekemia cell)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 09 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Human Cell lysate - whole cell (293T (human kidney cell))
Specification 293T (human kidney cell)
Total protein in input 25 µg
Username

Abcam user community

Verified customer

Submitted Jul 05 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"