Anti-HA tag antibody [HA.C5] (ab18181)
- Product nameAnti-HA tag antibody [HA.C5]See all HA tag primary antibodies ...
- DescriptionMouse monoclonal [HA.C5] to HA tag
- SpecificityRecognizes HA-tagged proteins overexpressed in cells, including both amino- and carboxy-termini of targeted proteins in transfected mammalian cells.
- Tested applicationsChIP/Chip, WB, ICC, IP, ICC/IF more details
Synthetic peptide from influenza hemagglutinin epitope: YPYDVPDYA conjugated to KLH.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: PBS, pH 7.4
- Concentration information loading...
- PurityIgG fraction
- Clonality Monoclonal
- Clone numberHA.C5
- Research Areas
Our Abpromise guarantee covers the use of ab18181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP/Chip||ChIP/Chip: Use at an assay dependent dilution.|
|IP||IP: Use at an assay dependent dilution.|
|ICC/IF||ICC/IF: Use at an assay dependent dilution.|
- RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
- hemagglutinin antibody
- Hemagglutinin HA1 chain antibody
- Hemagglutinin HA2 chain antibody
Anti-HA tag antibody [HA.C5] images
All lanes : Anti-HA tag antibody [HA.C5] (ab18181) at 1/1000 dilution
Lane 1 : HEK293 whole cell lysate - transfected
Lane 2 : HEK293 whole cell lysate - transfected
Lane 3 : HEK293 whole cell lysate - untransfected
Lysates/proteins at 30 µg per lane.
IRDye® 800CW Goat anti-mouse IgG polyclonal at 1/10000 dilution
Performed under reducing conditions.
Observed band size : 85 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes
This image is courtesy of an anonymous Abreview
Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1). Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1).
Immunofluorescence using ab18181 staining a HA-tag fusion protein (transcription factor) in a stable expressing cell line (right hand panel) and control cell line (left hand panel).
All lanes : Anti-HA tag antibody [HA.C5] (ab18181) at 1/2000 dilution
Lane 1 : WCE from cell line transfected for HA-tagged protein
Lane 2 : WCE from a cell line transfected with empty vector
Lysates/proteins at 50 µg per lane.
HRP conjugated Goat anti-mouse IgG (H+L)
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds
This image is courtesy of an anonymous AbreviewIncubation with the primary antibody was carried out at 4°C overnight, whilst the secondary antibody was incubated for 1 hour at room temperature.
ab18181 staining HA tag (green) in HeLa cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 22°C. A FITC-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (blue).
References for Anti-HA tag antibody [HA.C5] (ab18181)
This product has been referenced in:
- van Kasteren PB et al. Deubiquitinase function of arterivirus papain-like protease 2 suppresses the innate immune response in infected host cells. Proc Natl Acad Sci U S A 110:E838-47 (2013). WB . Read more (PubMed: 23401522) »
- Lang UE et al. GSK3ß phosphorylation of the KLF6 tumor suppressor promotes its transactivation of p21. Oncogene 32:4557-64 (2013). Read more (PubMed: 23085750) »