Overview

  • Product name
    Anti-HA tag antibody [HA.C5]
    See all HA tag primary antibodies
  • Description
    Mouse monoclonal [HA.C5] to HA tag
  • Host species
    Mouse
  • Tested applications
    Suitable for: ChIP/Chip, WB, ICC, IP, ICC/IFmore details
  • Immunogen

    Synthetic peptide from influenza hemagglutinin epitope:

    YPYDVPDYA

    conjugated to KLH.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    Purified from TCS
  • Clonality
    Monoclonal
  • Clone number
    HA.C5
  • Isotype
    IgG3
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab18181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP/Chip Use at an assay dependent concentration. PubMed: 19581286
WB 1/1000.
ICC 1/200.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Images

  • All lanes : Anti-HA tag antibody [HA.C5] (ab18181) at 1/1000 dilution

    Lanes 1-2 : HEK293 whole cell lysate - transfected
    Lane 3 : HEK293 whole cell lysate - untransfected

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : IRDye® 800CW Goat anti-mouse IgG polyclonal at 1/10000 dilution

    Performed under reducing conditions.

    Observed band size: 85 kDa (why is the actual band size different from the predicted?)


    Exposure time: 5 minutes

    See Abreview

  • ab18181 staining HA tag (green) in HeLa cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 22°C. A FITC-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (blue).

    See Abreview

  • Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1). Western blot using ab18181 of 293 cells transfected with HA-tagged vector(2) and untransfected control (1).
  • Immunofluorescence using ab18181 staining a HA-tag fusion protein (transcription factor) in a stable expressing cell line (right hand panel) and control cell line (left hand panel).
  • All lanes : Anti-HA tag antibody [HA.C5] (ab18181) at 1/2000 dilution

    Lane 1 : WCE from cell line transfected for HA-tagged protein
    Lane 2 : WCE from a cell line transfected with empty vector

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP conjugated Goat anti-mouse IgG (H+L)

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 10 seconds


    Incubation with the primary antibody was carried out at 4°C overnight, whilst the secondary antibody was incubated for 1 hour at room temperature.

    See Abreview

References

This product has been referenced in:
  • Akiyama T  et al. Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing. Sci Rep 8:1189 (2018). Read more (PubMed: 29352121) »
  • Cawez F  et al. Combinatorial Design of a Nanobody that Specifically Targets Structured RNAs. J Mol Biol 430:1652-1670 (2018). Read more (PubMed: 29654796) »

See all 67 Publications for this product

Customer reviews and Q&As

Thank you for getting back to me. I am sorry that my previous e-mail has not reached you. This is to let you know that I have placed a new order for you - for one vial of ab18181 as a free of charge replacement (exchange for the original item: a...

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Thank you for your enquiry. NChIP involves the preparation of native, nuclease digested chromatin followed by the immunoprecipitation of chromatin using appropriate antibodies: We have an excellent protocol for both the preparation of nuclease di...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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