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Product Name
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HA tag antibody (HRP)
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See all HA tag antibodies (28)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to HA tag (HRP)
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Immunogen
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YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH. BLAST 'YPYDVPDYA' with or 
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Specificity
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In a direct ELISA against 1ug of HA peptide coupled to BSA as sample, conjugate diluted to 1/15,000 produced a 1.0 OD in a 15 minute reaction with TMB as substrate.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ELISA: Use at a concentration of 0.05 - 0.5 µg/ml. WB: Use at a concentration of 0.2 - 1 µg/ml.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Tags & Cell Markers >> Epitope Tags >> HA Tag
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Relevance
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Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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50 mM HEPES pH 7.0, 0.1 M NaCl with 0.2% BSA
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Purification notes
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Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase.
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Form
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Liquid
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Conjugation
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Horse Radish Peroxidase
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HA tag antibody (HRP) is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to HA tag
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