Overview

  • Product nameAnti-HA tag antibody (HRP)
    See all HA tag primary antibodies
  • Description
    Rabbit polyclonal to HA tag (HRP)
  • ConjugationHRP
  • SpecificityIn a direct ELISA against 1ug of HA peptide coupled to BSA as sample, conjugate diluted to 1/15,000 produced a 1.0 OD in a 15 minute reaction with TMB as substrate.
  • Tested applicationsELISA, WBmore details
  • Immunogen

    YPYDVPDYA

    (influenza hemagglutinin-HA-epitope) conjugated to KLH.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferpH: 7.00
    Preservative: 0.002% Thimerosal (merthiolate)
    Constituent: 1.19% HEPES
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab1188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.05 - 0.5 µg/ml.
WB Use a concentration of 0.2 - 1 µg/ml.

Target

  • RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • Alternative names
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

References for Anti-HA tag antibody (HRP) (ab1188)

This product has been referenced in:
  • Uriarte SM  et al. Granule exocytosis contributes to priming and activation of the human neutrophil respiratory burst. J Immunol 187:391-400 (2011). WB . Read more (PubMed: 21642540) »

See 1 Publication for this product

Product Wall

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I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (human)
Loading amount 20 µg
Specification human
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Apr 17 2007

Thank you for your email and patience. I have tried contacting the originator of this antibody but unfortunately have not heard back. I,m not sure why the antibody is not recognizing the HA tag in your other samples. What was your order number? If you ...

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Could we get a detailed protocol from you, please? We have some general questions, the answers to which will enable us to investigate this matter as quickly as possible. Also, please include the lot number of ab1188 that you received (it is located ...

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Thank you for your enquiry. All the information that we have regarding this antibody is located on the online datasheet. Unfortunately, we are not aware of any publications that feature the use of this antibody. If you have any more questions, do not h...

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There is no special protocol necessary for using our antibody in this system. As long as the HA tag is exposed, the antibody will be useful. We recommend a starting dilution of 1:1000.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"