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Product Name 

HA tag antibody (HRP)

 

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Product type 

Primary antibodies

Description 

Rabbit polyclonal to HA tag (HRP)

Immunogen 

YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH.
BLAST 'YPYDVPDYA' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Specificity 

In a direct ELISA against 1ug of HA peptide coupled to BSA as sample, conjugate diluted to 1/15,000 produced a 1.0 OD in a 15 minute reaction with TMB as substrate.

Tested applications 
(see key)

ELISA, WB


Abreviews 

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Application notes 
(see key)

Recommended dilutions
ELISA: Use at a concentration of 0.05 - 0.5 µg/ml.
WB: Use at a concentration of 0.2 - 1 µg/ml.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Tags & Cell Markers >> Epitope Tags >> HA Tag

Relevance 

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

 

Alternative names HA tag antibody (HRP) (ab1188)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

50 mM HEPES pH 7.0, 0.1 M NaCl with 0.2% BSA

Purification notes 

Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase.

Form 

Liquid

Conjugation 

Horse Radish Peroxidase

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HA tag antibody (HRP) is on this datasheet. But please do contact us if you would like any reassurance!


 

Search PubMed (MEDLINE) for references to HA tag

 

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HA tag antibody (HRP) - related products:

Products for Western blot

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Lysates

 

Positive Controls for HA tag antibody (HRP)

ab20896

  

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate - expressing HA tag protein

 

Other HA tag Antibodies

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