The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
HA epitope tag antibody
Hemagglutinin HA1 chain antibody
Hemagglutinin HA2 chain antibody
Western blot - Anti-HA tag antibody (HRP) (ab183884)
All lanes : Anti-HA tag antibody (HRP) (ab183884) at 1/3000 dilution
Lane 1 : Non-transfected HeLa cell lysate Lane 2 : Transfected HeLa cells over expressing HA-tagged protein lysate