Overview

  • Product nameHA tag peptide

Description

  • NatureSynthetic
  • Amino Acid Sequence

    Specifications

    Our Abpromise guarantee covers the use of ab13835 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Blocking - Blocking peptide for Anti-HA tag antibody (ab13834)

    • FormLiquid
    • Additional notes

      - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
      - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
      - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
      - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
      - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      Information available upon request.

    General Info

    • Alternative names
      • HA epitope tag
      • HA1
      • HA2
      • hemagglutinin
      • Hemagglutinin HA1 chain
      • Hemagglutinin HA2 chain
      see all
    • RelevanceHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    HA tag peptide images

    • All lanes : Anti-HA tag antibody (ab13834) at 1/500 dilution

      Lane 1 : HEK293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896)
      Lane 2 : HEK293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896) with HA tag peptide (ab13835) at 1 µg/ml

      Lysates/proteins at 10 µg per lane.

      Secondary
      Alexa Fluor 680 Goat-anti-Rabbit IgG at 1/5000 dilution

      Performed under reducing conditions.

      Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    References for HA tag peptide (ab13835)

    ab13835 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting Abcam regarding ab13835.


    This product is the HA peptide used as the immunogen to generate the antibody ab13834. Since it is only 9aa, it cannot be run on a gel to use as a positive control or standard. Instead, I ...

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    Thank you for contacting us.
    Yes, we expect that antibody to bind this peptide. The peptide ab13835 does contain the classical HA tag (amino acid 98 to 106 of the surface hemagglutinin glycoprotein of Human influenza virus). The cited antibody is ...

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    Here is a general protocol for eluting your HA-tagged protein with ab13835, our HA tag peptide: The peptide arrives at a 1mg/ml concentration. After the last wash, remove all of the wash buffer and add a sufficient volume of the peptide for your dow...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"