Overview

  • Product nameAnti-HABP2 antibody
    See all HABP2 primary antibodies
  • Description
    Rabbit polyclonal to HABP2
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 288-337 (PTEPSTKLPG FDSCGKTEIA ERKIKRIYGG FKSTAGKHPW QASLQSSLPL) of human HABP2 (NP_004123).

  • Positive control
    • MCF7 cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab81490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml.
ELISA Use at an assay dependent concentration.

Target

  • FunctionCleaves the alpha-chain at multiple sites and the beta-chain between 'Lys-53' and 'Lys-54' but not the gamma-chain of fibrinogen and therefore does not initiate the formation of the fibrin clot and does not cause the fibrinolysis directly. It does not cleave (activate) prothrombin and plasminogen but converts the inactive single chain urinary plasminogen activator (pro-urokinase) to the active two chain form. Activates coagulation factor VII.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 3 EGF-like domains.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    Proteolytic cleavage at Gly-23 or Met-27 can give rise to the 50 kDa heavy chain and cleavage at Arg-313 or Lys-319 can give rise to the 27 kDa light chain. The heavy chain can undergo further proteolytic cleavage at Lys-169 or Arg-170 to give rise to 2 inactive 26 kDa fragments and the light chain can undergo further proteolytic cleavage at Arg-480 to give rise to inactive 17 kDa and 8 kDa fragments.
  • Cellular localizationSecreted. Secreted as an inactive single-chain precursor and is then activated to a heterodimeric form.
  • Information by UniProt
  • Database links
  • Alternative names
    • Factor seven activating protease antibody
    • Factor seven-activating protease antibody
    • Factor VII activating protein antibody
    • Factor VII-activating protease antibody
    • FSAP antibody
    • HABP 2 antibody
    • HABP antibody
    • Habp2 antibody
    • HABP2_HUMAN antibody
    • Hepatocyte growth factor activator like protein antibody
    • Hepatocyte growth factor activator-like protein antibody
    • HGFAL antibody
    • Hyaluronan binding protein 2 antibody
    • Hyaluronan-binding protein 2 27 kDa light chain alternate form antibody
    • Hyaluronic acid binding protein 2 antibody
    • PHBP antibody
    • Plasma hyaluronan binding protein antibody
    • Plasma hyaluronan-binding protein antibody
    see all

Anti-HABP2 antibody images

  • Anti-HABP2 antibody (ab81490) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 63 kDa
    Observed band size : 63 kDa
    Additional bands at : 27 kDa,28 kDa. We are unsure as to the identity of these extra bands.
  • IHC image of HABP2 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab81490, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-HABP2 antibody (ab81490)

This product has been referenced in:
  • Jiang F  et al. Serum Proteome Changes in Healthy Subjects with Different Genotypes of NOS1AP in the Chinese Population. J Diabetes Res 2013:357630 (2013). WB ; Human . Read more (PubMed: 23671866) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"