Recombinant Anti-HABP2 antibody [EPR14551] (ab181837)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14551] to HABP2
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-HABP2 antibody [EPR14551]
See all HABP2 primary antibodies -
Description
Rabbit monoclonal [EPR14551] to HABP2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- ICC/IF: MCF7. Flow Cyt (intra): HepG2. WB: MCF2 and A549 whole cell lysate. Mouse brain and heart lysate. Rate brain and heart lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14551 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181837 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/20.
|
|
WB | (1) |
1/10000. Predicted molecular weight: 63 kDa.
For unpurified use at 1/10000 - 1/50000. |
ICC/IF |
1/50.
For unpurified use at 1/100. |
Notes |
---|
Flow Cyt (Intra)
1/20. |
WB
1/10000. Predicted molecular weight: 63 kDa. For unpurified use at 1/10000 - 1/50000. |
ICC/IF
1/50. For unpurified use at 1/100. |
Target
-
Function
Cleaves the alpha-chain at multiple sites and the beta-chain between 'Lys-53' and 'Lys-54' but not the gamma-chain of fibrinogen and therefore does not initiate the formation of the fibrin clot and does not cause the fibrinolysis directly. It does not cleave (activate) prothrombin and plasminogen but converts the inactive single chain urinary plasminogen activator (pro-urokinase) to the active two chain form. Activates coagulation factor VII. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 3 EGF-like domains.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsProteolytic cleavage at Gly-23 or Met-27 can give rise to the 50 kDa heavy chain and cleavage at Arg-313 or Lys-319 can give rise to the 27 kDa light chain. The heavy chain can undergo further proteolytic cleavage at Lys-169 or Arg-170 to give rise to 2 inactive 26 kDa fragments and the light chain can undergo further proteolytic cleavage at Arg-480 to give rise to inactive 17 kDa and 8 kDa fragments. -
Cellular localization
Secreted. Secreted as an inactive single-chain precursor and is then activated to a heterodimeric form. - Information by UniProt
-
Database links
- Entrez Gene: 3026 Human
- Entrez Gene: 226243 Mouse
- Entrez Gene: 292126 Rat
- Omim: 603924 Human
- SwissProt: Q14520 Human
- SwissProt: Q8K0D2 Mouse
- SwissProt: Q6L711 Rat
- Unigene: 422542 Human
see all -
Alternative names
- Factor seven activating protease antibody
- Factor seven-activating protease antibody
- Factor VII activating protein antibody
see all
Images
-
All lanes : Anti-HABP2 antibody [EPR14551] (ab181837) at 1/10000 dilution (Purified)
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
Lane 3 : Mouse brain lysate
Lane 4 : Mouse heart lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
Immunocytochemistry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling HABP2 with purified ab181837 at 1/50 dilution (2.46 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
-
Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling HABP2 with purified ab181837 at 1/20 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (3)
ab181837 has been referenced in 3 publications.
- Hong Y et al. Degradation of tumour stromal hyaluronan by small extracellular vesicle-PH20 stimulates CD103+ dendritic cells and in combination with PD-L1 blockade boosts anti-tumour immunity. J Extracell Vesicles 8:1670893 (2019). PubMed: 31632619
- Colombo C et al. Segregation and expression analyses of hyaluronan-binding protein 2 (HABP2): insights from a large series of familial non-medullary thyroid cancers and literature review. Clin Endocrinol (Oxf) 86:837-844 (2017). PubMed: 28222214
- Ichimaru S et al. Hypoxia Potentiates Anabolic Effects of Exogenous Hyaluronic Acid in Rat Articular Cartilage. Int J Mol Sci 17:N/A (2016). PubMed: 27347945