Anti-HADHA/HADHB antibody [4A8BG12] (ab110302)


  • Product nameAnti-HADHA/HADHB antibody [4A8BG12]
  • Description
    Mouse monoclonal [4A8BG12] to HADHA/HADHB
  • Tested applicationsSuitable for: ICC, IHC-P, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Rat, Cow, Human
  • Immunogen

    Human heart mitochondria

  • Positive control
    • HDFn and HL60 cells; HepG2 cell lysate; Human cerebellum tissue.
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.


Associated products


Our Abpromise guarantee covers the use of ab110302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration. 0.5-2mg protein/10ul antibody


  • PathwayLipid metabolism; fatty acid beta-oxidation.
  • Involvement in diseaseDefects in HADHB are a cause of trifunctional protein deficiency (TFP deficiency) [MIM:609015]. The clinical manifestations are very variable and include hypoglycemia, cardiomyopathy and sudden death. Phenotypes with mainly hepatic and neuromyopathic involvement can also be distinguished. Biochemically, TFP deficiency is defined by the loss of all three enzyme activities of the TFP complex.
  • Sequence similaritiesBelongs to the thiolase family.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3-ketoacyl-CoA thiolase antibody
    • 4930479F15Rik antibody
    • Acetyl-CoA acyltransferase antibody
    • Beta-ketothiolase antibody
    • C77020 antibody
    • ECHA antibody
    • ECHB antibody
    • ECHB_HUMAN antibody
    • GBP antibody
    • HADH antibody
    • Hadhb antibody
    • LCEH antibody
    • LCHAD antibody
    • MGC105338 antibody
    • MGC1728 antibody
    • MGC87480 antibody
    • MS734 antibody
    • MSTP029 antibody
    • MTPA antibody
    • MTPB antibody
    • RGD1560655 antibody
    • TP ALPHA antibody
    • TP-beta antibody
    • Trifunctional enzyme subunit alpha, mitochondrial antibody
    see all

Anti-HADHA/HADHB antibody [4A8BG12] images

  • Immunocytochemistry analysis using ab110302 at 5µg/ml staining HADHA/HADHB in HDFn cells.
  • Flow cytometric analysis using ab110302 at 1µg/ml staining HADHA/HADHB in HL60 cells (blue). Isotype control antibody (red).
  • Detection of ab110302 by Western Blot of Immunprecipitate.
    Staining of HADHA/HADHB in 1 mg HepG2 cell lysate/10ul antibody conjugated beads immunoprecipitated using ab110302.
  • Immunohistochemistry analysis using ab110302 staining HADHA/HADHB in Human cerebellum Formalin-fixed, Paraffin-embedded tissue.

References for Anti-HADHA/HADHB antibody [4A8BG12] (ab110302)

ab110302 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Heart)
Permeabilization Yes - Tween-20
Specification Heart
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 29 2016

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Mouse Tissue lysate - whole (Heart)
Specification Heart
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Ms. Arcan Guven

Verified customer

Submitted Sep 03 2014

Thank you very much for your telephone call this morning. As discussed, to our knowledge I am sorry to confirm that ab110302 or ab110281 have not been tested in rabbit samples. Both these antibodies have been raised against the native hum...

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