Overview

  • Product name
  • Description
    Chicken polyclonal to HAGH
  • Specificity
    ab37432 recognises hydroxyacylglutathione hydrolase
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to N terminal amino acids 110-260 of Human HAGH.

Properties

Applications

Our Abpromise guarantee covers the use of ab37432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28.9 kDa.
ELISA 1/2000.

Target

  • Relevance
    Hydroxyacylglutathione hydrolase (HAGH) is a thiolesterase which hydrolyses S-lactoyl-glutathione to reduced glutathione and D-lactate.
  • Cellular localization
    Cytoplasmic and Mitochondrial
  • Database links
  • Alternative names
    • GLO2 antibody
    • GLX II antibody
    • Glyoxalase II antibody
    • HAGH antibody
    • HAGH1 antibody
    • Hydroxyacyl glutathione hydrolase antibody
    • Hydroxyacylglutathione hydrolase, mitochondrial antibody
    see all

Images

  • Direct ELISA using protein as test antigen (serially diluted). Primary antibody is ab37432 and secondary is Goat anti-IgY HRP (1 ug/ml).
  • Anti-HAGH antibody (ab37432) at 1/2000 dilution (E coli-derived fusion protein)

    Secondary
    Goat anti-IgY-HRP

    Predicted band size : 28.9 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Kim ST  et al. The effect of electroaucpuncture for 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced proteomic changes in the mouse striatum. J Physiol Sci 60:27-34 (2010). WB ; Mouse . Read more (PubMed: 19760485) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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