Anti-hair cortex Cytokeratin antibody (ab112444)

Overview

  • Product nameAnti-hair cortex Cytokeratin antibody
    See all hair cortex Cytokeratin primary antibodies
  • Description
    Rabbit polyclonal to hair cortex Cytokeratin
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human hair cortex Cytokeratin aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q6A162

  • Positive control
    • This antibody gave a positive signal in the following Human whole cell lysates: Caco2; MCF7; SW480; LOVO. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal skin.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab112444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

Target

  • FunctionMay play a role in late hair differentiation.
  • Tissue specificityExpressed in skin and scalp. Also very weakly expressed in tongue, breast, colon and small intestine. In the hair follicle, it is specifically present in the upper hair cuticle. Not present in the upper cortex (at protein level).
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Developmental stageDuring differentiation of the hair, it is one of the last keratins expressed.
  • Information by UniProt
  • Database links
  • Alternative names
    • CK-40 antibody
    • Cytokeratin-40 antibody
    • K1C40_HUMAN antibody
    • K40 antibody
    • Keratin antibody
    • Keratin, type I cytoskeletal 40 antibody
    • Keratin-40 antibody
    • Krt40 antibody
    • type I cytoskeletal 40 antibody
    • Type I hair keratin Ka36 antibody
    see all

Anti-hair cortex Cytokeratin antibody images

  • IHC image of hair cortex Cytokeratin staining in Human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112444, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • All lanes : Anti-hair cortex Cytokeratin antibody (ab112444) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 48 kDa


    Exposure time : 2 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab112444 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References for Anti-hair cortex Cytokeratin antibody (ab112444)

ab112444 has not yet been referenced specifically in any publications.

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