Anti-hair cortex Cytokeratin antibody [AE13] (ab16113)

Overview

  • Product nameAnti-hair cortex Cytokeratin antibody [AE13]
    See all hair cortex Cytokeratin primary antibodies
  • Description
    Mouse monoclonal [AE13] to hair cortex Cytokeratin
  • SpecificityThis antibody is specific for the family of hair cortex keratins.
  • Tested applicationsSuitable for: IHC-P, WB, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Human hair keratins.

  • General notes


    This antibody is an excellent marker for hair and nail differentiation.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesThis antibody is an excellent marker for hair and nail differentiation.
  • ClonalityMonoclonal
  • Clone numberAE13
  • MyelomaP3x63-Ag8.653
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/1000 - 1/3000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 - 46 kDa).
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/20 - 1/50. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionMay play a role in late hair differentiation.
  • Tissue specificityExpressed in skin and scalp. Also very weakly expressed in tongue, breast, colon and small intestine. In the hair follicle, it is specifically present in the upper hair cuticle. Not present in the upper cortex (at protein level).
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Developmental stageDuring differentiation of the hair, it is one of the last keratins expressed.
  • Information by UniProt
  • Database links
  • Alternative names
    • CK-40 antibody
    • Cytokeratin-40 antibody
    • K1C40_HUMAN antibody
    • K40 antibody
    • Keratin antibody
    • Keratin, type I cytoskeletal 40 antibody
    • Keratin-40 antibody
    • Krt40 antibody
    • type I cytoskeletal 40 antibody
    • Type I hair keratin Ka36 antibody
    see all

Anti-hair cortex Cytokeratin antibody [AE13] images

  • ab16113 at 1/100 staining mouse skin tissue sections by IHC-P. The tissue was paraformaldehyde fixed and blocked with BSA. A heat mediated antogen retrieval step was performed. The tissue was incubated with the antibody for 16 hours and then an Alexa-Fluor 488 conjugated goat anti-mouse antibody was used as the secondary. The image shows hair cortex cytokeratin staining in green with DAPI nuclei counterstain in blue.

    See Abreview

  • ab16113 staining hair cortex Cytokeratin in adult mouse nail by Immunohistochemistry (paraffin embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C followed by incubation with the primary antibody, at a 1/200 dilution, for 12 hours at 4°C. A Biotin-conjugated goat anti-mouse monoclonal was used as secondary antibody at a 1/400 dilution.

    See Abreview

  • All lanes : Anti-hair cortex Cytokeratin antibody [AE13] (ab16113) at 1 µg/ml

    Lane 1 : Mouse skin tissue lysate (14 days) (ab7271)
    Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 - 46 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa,55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • Overlay histogram showing A431 cells stained with ab16113 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16113, 1/20 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

References for Anti-hair cortex Cytokeratin antibody [AE13] (ab16113)

This product has been referenced in:
  • Yuan S  et al. Post-transcriptional Regulation of Keratinocyte Progenitor Cell Expansion, Differentiation and Hair Follicle Regression by miR-22. PLoS Genet 11:e1005253 (2015). Read more (PubMed: 26020521) »
  • Wang D  et al. MicroRNA-205 controls neonatal expansion of skin stem cells by modulating the PI(3)K pathway. Nat Cell Biol 15:1153-63 (2013). Read more (PubMed: 23974039) »

See all 10 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Skin)
Permeabilization No
Specification Skin
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Ahmar Aziz

Verified customer

Submitted Jul 08 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (skin)
Specification skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: H-3300 Vector lab
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 01 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Adult nail)
Specification Adult nail
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Mar 25 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (skin)
Specification skin
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Username

Abcam user community

Verified customer

Submitted Mar 09 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"