This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Overview

Properties

Applications

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

Application Abreviews Notes
ICC/IF 1/150. This Fast-track antibody has been successfully used for ICC/IF using rat oligodendrocyte cell culture. However, please be aware that this information should be used for information purposes only, and that Abcam cannot guarantee that all batches of this antibody will be equally suitable.
WB Use a concentration of 5 µg/ml. This Fast-track antibody has been successfully used for WB using rat brain tissue lysates. However, please be aware that this information should be used for information purposes only, and that Abcam cannot guarantee that all batches of this antibody will be equally suitable.
ELISA Use at an assay dependent concentration. : This antibody gave a positive result in ELISA against the immunizing peptide (ab37539).
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. This Fast-track antibody has been successfully used for IHC-P on oral mucosa tissue. However, please be aware that this information should be used for information purposes only, and that Abcam cannot guarantee that all batches of this antibody will be equally suitable.

Target

  • FunctionMay act as a transcription factor that could act on to regulate one of the phases of hair growth.
  • Tissue specificityStrongest expression of isoforms 1 and 2 is seen in the small intestine, weaker expression in brain and colon, and trace expression is found in liver, pancreas, spleen, thymus, stomach, salivary gland, appendix and trachea. Isoform 1 is always the most abundant. Isoform 1 is exclusively expressed at low levels in kidney and testis. Isoform 2 is exclusively expressed at high levels in the skin.
  • Involvement in diseaseDefects in HR are the cause of alopecia universalis congenita (ALUNC) [MIM:203655]. ALUNC is a rare autosomal recessive form of hair loss characterized by hair follicles without hair.
    Defects in HR are the cause of atrichia with papular lesions (APL) [MIM:209500]; also known as congenital atrichia. APL is an autosomal recessive disease characterized by papillary lesions over most of the body and almost complete absence of hair.
    Defects in HR are the cause of hypotrichosis Marie Unna type 1 (MUHH1) [MIM:146550]. An autosomal dominant condition characterized by reduced amount of hair, alopecia, little or no eyebrows, eyelashes or body hair, and coarse, wiry, twisted hair in early childhood.
  • Sequence similaritiesContains 1 JmjC domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALUNC antibody
    • AU antibody
    • HAIR_HUMAN antibody
    • Hairless protein antibody
    • Host range antibody
    • HR antibody
    • HSA277165 antibody
    • Protein hairless antibody
    see all

References for Anti-Hairless antibody (ab37540)

ab37540 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (Brain)
Loading amount 30 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (12% Novex Gels)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Oct 11 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Oligodendrocyte)
Specification Oligodendrocyte
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% triton X-100
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
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Submitted Jul 08 2011

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Oral mucosa)
Specification Oral mucosa
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric buffer, pH6
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
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Submitted Jun 07 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (P19)
Specification P19
Fixative Formaldehyde
Permeabilization Yes - 0.2% Triton X 100
Blocking step Serum as blocking agent for 2 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 26°C
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Submitted Mar 26 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"