The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC: This antibody may be diluted to a titer of 1:15-1:30 in an ABC method.
Staining Protocol: We suggest an incubation period of 60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
Enzymatic antigen retrieval of formalin fixed paraffin embedded tissue sections is required prior to immunostaining.
Formalin fixed paraffin embedded human lymph node stained with Hairy Cell Leukemia using ABC and AEC chromogen.
References for Anti-Hairy Cell Leukemia antibody [DBA.44] (ab779)
This product has been referenced in:
al Saati T et al. Production of anti-B monoclonal antibodies (DBB.42, DBA.44, DNA.7, and DND.53) reactive on paraffin-embedded tissues with a new B-lymphoma cell line grafted into athymic nude mice. Blood74:2476-85 (1989).
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