Anti-HAND1 antibody (ab104320)

Overview

  • Product name
  • Description
    Rabbit polyclonal to HAND1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 35-84 (CHQERPYFQS WLLSPADAAP DFPAGGPPPA AAAAATAYGP DARPGQSPGR) of Human HAND1 (NP_004812).

  • Positive control
    • Human fetal liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab104320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box).
  • Tissue specificity
    Heart.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications
    Phosphorylation by PLK4 disrupts the interaction with MDFIC and leads to tranlocation into the nucleoplasm, allowing dimerization and transcription factor activity.
  • Cellular localization
    Nucleus > nucleoplasm. Nucleus > nucleolus. Interaction with MDFIC sequesters it into the nucleolus, preventing the transcription factor activity. Phosphorylation by PLK4 disrupts the interaction with MDFIC and releases it from the nucleolus, leading to transcription factor activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • autonomic nervous system and neural crest derivatives-expressed protein 1 antibody
    • Basic helix loop helix transcription factor HAND1 antibody
    • bHLHa27 antibody
    • Class A basic helix-loop-helix protein 27 antibody
    • eHAND antibody
    • Extraembryonic tissues antibody
    • Extraembryonic tissues heart autonomic nervous system and neural crest derivatives expressed protein 1 antibody
    • HAND 1 antibody
    • HAND1 antibody
    • HAND1_HUMAN antibody
    • Heart and neural crest derivatives expressed 1 antibody
    • Heart and neural crest derivatives expressed protein 1 antibody
    • heart antibody
    • Heart- and neural crest derivatives-expressed protein 1 antibody
    • Hxt antibody
    • Thing 1 antibody
    • Thing1 antibody
    • Thing1 antibody
    see all

Images

  • Anti-HAND1 antibody (ab104320) at 1 µg/ml + Human fetal liver lysate at 10 µg

    Predicted band size : 24 kDa

References

ab104320 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Non-reduced Denaturing
Sample
Mouse Tissue lysate - whole (293T transfected with Flag-mHand1)
Specification
293T transfected with Flag-mHand1
Treatment
Drug X treated for 48 h
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Hongfeng Ruan

Verified customer

Submitted Jan 08 2015

Thank you for your inquiry.

We have a number of antibodies in our catalog that might be of interest to you. I would like to direct you to the search facility on our website (www.abcam.com).

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