- Datasheet
- Specific References
- Protocols
Overview
-
Product nameAnti-HAND1 antibody
See all HAND1 primary antibodies -
DescriptionRabbit polyclonal to HAND1
-
Host speciesRabbit
-
Tested applicationsSuitable for: ELISA, IHC-Pmore details
-
Species reactivityReacts with: Human
Predicted to work with: Rat, Sheep, Rabbit, Horse, Cow, Dog, Pig
-
Immunogen
Synthetic peptide:
QHEGFPPALGPVEK
conjugated to KLH, corresponding to amino acids 183-196 of Human HAND1 -
Positive control
- Human Heart tissue
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
-
Storage bufferPreservative: 0.01% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
PurityProtein G purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab115256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/1 - 1/1000. | |
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
FunctionTranscription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box).
-
Tissue specificityHeart.
-
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
-
Post-translational
modificationsPhosphorylation by PLK4 disrupts the interaction with MDFIC and leads to tranlocation into the nucleoplasm, allowing dimerization and transcription factor activity. -
Cellular localizationNucleus > nucleoplasm. Nucleus > nucleolus. Interaction with MDFIC sequesters it into the nucleolus, preventing the transcription factor activity. Phosphorylation by PLK4 disrupts the interaction with MDFIC and releases it from the nucleolus, leading to transcription factor activity.
- Information by UniProt
-
Database links
- Entrez Gene: 9421 Human
- Entrez Gene: 59112 Rat
- Omim: 602406 Human
- SwissProt: O96004 Human
- SwissProt: P57100 Rabbit
- SwissProt: P97832 Rat
- SwissProt: Q28555 Sheep
- Unigene: 152531 Human
see all -
Alternative names
- autonomic nervous system and neural crest derivatives-expressed protein 1 antibody
- Basic helix loop helix transcription factor HAND1 antibody
- bHLHa27 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAND1 antibody (ab115256)Human Heart (formalin-fixed, paraffin-embedded) stained with ab115256 at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Protocols
References
ab115256 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"