Anti-HAND1 antibody (ab115256)

Overview

• Product name
  Anti-HAND1 antibody
  See all HAND1 primary antibodies
• Description
  Rabbit polyclonal to HAND1
• Tested applications
  Suitable for: ELISA, IHC-Pmore details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Rat, Sheep, Rabbit, Horse, Cow, Dog, Pig
• Immunogen

  Synthetic peptide:

  QHEGFPPALGPVEK

  conjugated to KLH, corresponding to amino acids 183-196 of Human HAND1

  Run BLAST with Run BLAST with
• Positive control
  • Human Heart tissue

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
• Storage buffer
  Preservative: 0.01% Sodium azide
  Constituent: 99% PBS
• Concentration information loading...
• Purity
  Protein G purified
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Stem Cells
  • Lineage Markers
  • Ectoderm

  • Cardiovascular
  • Heart
  • Cardiogenesis
  • Transcription factors/regulators

  • Developmental Biology
  • Lineage specification
  • Ectoderm

Associated products

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

Applications

Target

• Function
  Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box).
• Tissue specificity
  Heart.
• Sequence similarities
  Contains 1 basic helix-loop-helix (bHLH) domain.
• Post-translational
  modifications
  Phosphorylation by PLK4 disrupts the interaction with MDFIC and leads to tranlocation into the nucleoplasm, allowing dimerization and transcription factor activity.
• Cellular localization
  Nucleus > nucleoplasm. Nucleus > nucleolus. Interaction with MDFIC sequesters it into the nucleolus, preventing the transcription factor activity. Phosphorylation by PLK4 disrupts the interaction with MDFIC and releases it from the nucleolus, leading to transcription factor activity.

• Target information above from: UniProt accession O96004 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 9421 Human
  • Entrez Gene: 59112 Rat
  • Omim: 602406 Human
  • SwissProt: O96004 Human
  • SwissProt: P57100 Rabbit
  • SwissProt: P97832 Rat
  • SwissProt: Q28555 Sheep
  • Unigene: 152531 Human

  • Unigene: 224598 Rat
  • Unigene: 39340 Rat

  see all
• Alternative names
  • autonomic nervous system and neural crest derivatives-expressed protein 1 antibody
  • Basic helix loop helix transcription factor HAND1 antibody
  • bHLHa27 antibody

  • Class A basic helix-loop-helix protein 27 antibody
  • eHAND antibody
  • Extraembryonic tissues antibody
  • Extraembryonic tissues heart autonomic nervous system and neural crest derivatives expressed protein 1 antibody
  • HAND 1 antibody
  • HAND1 antibody
  • HAND1_HUMAN antibody
  • Heart and neural crest derivatives expressed 1 antibody
  • Heart and neural crest derivatives expressed protein 1 antibody
  • heart antibody
  • Heart- and neural crest derivatives-expressed protein 1 antibody
  • Hxt antibody
  • Thing 1 antibody
  • Thing1 antibody
  • Thing1 antibody

  see all

Protocols

• Immunohistochemistry protocols