Overview

  • Product name
  • Description
    Rabbit polyclonal to HAND1
  • Tested applications
    Suitable for: ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    QHEGFPPALGPVEK

    conjugated to KLH, corresponding to amino acids 183-196 of Human HAND1

  • Positive control
    • Human Heart tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab115256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1 - 1/1000.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box).
  • Tissue specificity
    Heart.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications
    Phosphorylation by PLK4 disrupts the interaction with MDFIC and leads to tranlocation into the nucleoplasm, allowing dimerization and transcription factor activity.
  • Cellular localization
    Nucleus > nucleoplasm. Nucleus > nucleolus. Interaction with MDFIC sequesters it into the nucleolus, preventing the transcription factor activity. Phosphorylation by PLK4 disrupts the interaction with MDFIC and releases it from the nucleolus, leading to transcription factor activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • autonomic nervous system and neural crest derivatives-expressed protein 1 antibody
    • Basic helix loop helix transcription factor HAND1 antibody
    • bHLHa27 antibody
    • Class A basic helix-loop-helix protein 27 antibody
    • eHAND antibody
    • Extraembryonic tissues antibody
    • Extraembryonic tissues heart autonomic nervous system and neural crest derivatives expressed protein 1 antibody
    • HAND 1 antibody
    • HAND1 antibody
    • HAND1_HUMAN antibody
    • Heart and neural crest derivatives expressed 1 antibody
    • Heart and neural crest derivatives expressed protein 1 antibody
    • heart antibody
    • Heart- and neural crest derivatives-expressed protein 1 antibody
    • Hxt antibody
    • Thing 1 antibody
    • Thing1 antibody
    • Thing1 antibody
    see all

Anti-HAND1 antibody images

  • Human Heart (formalin-fixed, paraffin-embedded) stained with ab115256 at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-HAND1 antibody (ab115256)

ab115256 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Pig Tissue lysate - whole (Total heart)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Total heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Sep 29 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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