Anti-HAND1 antibody (ab46822)

Overview

  • Product name
  • Description
    Rabbit polyclonal to HAND1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Chicken, Cow, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human HAND1.

    (Peptide available as ab47013.)

  • Positive control
    • This antibody gave a positive signal in the following Human tissue lysates: Brain, Heart.

Properties

Applications

Our Abpromise guarantee covers the use of ab46822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25.8 kDa (predicted molecular weight: 25.8 kDa).

Target

  • Function
    Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box).
  • Tissue specificity
    Heart.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications
    Phosphorylation by PLK4 disrupts the interaction with MDFIC and leads to tranlocation into the nucleoplasm, allowing dimerization and transcription factor activity.
  • Cellular localization
    Nucleus > nucleoplasm. Nucleus > nucleolus. Interaction with MDFIC sequesters it into the nucleolus, preventing the transcription factor activity. Phosphorylation by PLK4 disrupts the interaction with MDFIC and releases it from the nucleolus, leading to transcription factor activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • autonomic nervous system and neural crest derivatives-expressed protein 1 antibody
    • Basic helix loop helix transcription factor HAND1 antibody
    • bHLHa27 antibody
    • Class A basic helix-loop-helix protein 27 antibody
    • eHAND antibody
    • Extraembryonic tissues antibody
    • Extraembryonic tissues heart autonomic nervous system and neural crest derivatives expressed protein 1 antibody
    • HAND 1 antibody
    • HAND1 antibody
    • HAND1_HUMAN antibody
    • Heart and neural crest derivatives expressed 1 antibody
    • Heart and neural crest derivatives expressed protein 1 antibody
    • heart antibody
    • Heart- and neural crest derivatives-expressed protein 1 antibody
    • Hxt antibody
    • Thing 1 antibody
    • Thing1 antibody
    • Thing1 antibody
    see all

Images

  • All lanes : Anti-HAND1 antibody (ab46822) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 25.8 kDa
    Observed band size : 25.8 kDa
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

References

ab46822 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Tissue lysate - whole (293T)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
60 µg
Treatment
transfected with pCMV-HAND1
Specification
293T
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Dec 18 2015

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (heart)
Specification
heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH9.0
Permeabilization
No
Blocking step
5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Nov 06 2008

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