Overview

  • Product name
  • Description
    Rabbit polyclonal to HAND2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region between amino acids 190-204 of Human HAND2.

  • Positive control
    • HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab10131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 26 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Essential for cardiac morphogenesis, particularly for the formation of the right ventricle and of the aortic arch arteries. Required for vascular development and regulation of angiogenesis, possibly through a VEGF signaling pathway. Plays also an important role in limb development, particularly in the establishment of anterior-posterior polarization, acting as an upstream regulator of sonic hedgehog (SHH) induction in the limb bud. Is involved in the development of branchial arches, which give rise to unique structures in the head and neck. Binds DNA on E-box consensus sequence 5'-CANNTG-3'.
  • Tissue specificity
    Heart.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Developmental stage
    Expressed in the fetal heart.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI225906 antibody
    • AI661148 antibody
    • autonomic nervous system and neural crest derivatives-expressed protein 2 antibody
    • Basic helix loop helix transcription factor HAND2 antibody
    • bHLHa26 antibody
    • Class A basic helix-loop-helix protein 26 antibody
    • Deciduum antibody
    • Deciduum heart autonomic nervous system and neural crest derivatives expressed protein 2 antibody
    • DHAND 2 antibody
    • dHAND antibody
    • DHAND2 antibody
    • Ehand 2 antibody
    • Ehand2 antibody
    • FLJ16260 antibody
    • HAND 2 antibody
    • Hand2 antibody
    • HAND2_HUMAN antibody
    • Heart and neural crest derivatives expressed 2 antibody
    • Heart and neural crest derivatives expressed protein 2 antibody
    • heart and neural crest derivatives expressed transcript 2 antibody
    • heart antibody
    • Heart- and neural crest derivatives-expressed protein 2 antibody
    • Hed antibody
    • Highly similar to dHAND M musculus antibody
    • MGC125303 antibody
    • MGC125304 antibody
    • Th 2 antibody
    • Th2 antibody
    • Thing 2 antibody
    • Thing2 antibody
    see all

Images



  • Predicted band size : 26 kDa


    ab10131 at 1/1000 dilution staining approximately 24kDa band of human HAND2 in HeLa whole cell lysate by Western blot (ECL).

References

This product has been referenced in:
  • Tang X  et al. Systemic morphine treatment derails normal uterine receptivity, leading to embryo implantation failure in mice. Biol Reprod 92:118 (2015). Read more (PubMed: 25855262) »

See 1 Publication for this product

Customer reviews and Q&As

Application
IHC - Wholemount
Sample
Zebrafish Embryo (wholemount)
Specification
wholemount
Username

Abcam user community

Verified customer

Submitted Dec 04 2013

Thank you for contacting us.

We have 3 antibodies available in Abcam catalogue; the numbers are ab10131, ab56590 and ab60037. Please choose any one of these for testing.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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