Anti-HAND2 antibody - Carboxyterminal end (ab60037)

Overview

  • Product name
    Anti-HAND2 antibody - Carboxyterminal end
    See all HAND2 primary antibodies
  • Description
    Rabbit polyclonal to HAND2 - Carboxyterminal end
  • Tested applications
    Suitable for: ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (10-30 aa in length) at the C-terminal of last 50 aa of human HAND2 conjugated to KLH

  • Positive control
    • Human hepatocarcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab60037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.

Target

  • Function
    Essential for cardiac morphogenesis, particularly for the formation of the right ventricle and of the aortic arch arteries. Required for vascular development and regulation of angiogenesis, possibly through a VEGF signaling pathway. Plays also an important role in limb development, particularly in the establishment of anterior-posterior polarization, acting as an upstream regulator of sonic hedgehog (SHH) induction in the limb bud. Is involved in the development of branchial arches, which give rise to unique structures in the head and neck. Binds DNA on E-box consensus sequence 5'-CANNTG-3'.
  • Tissue specificity
    Heart.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Developmental stage
    Expressed in the fetal heart.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI225906 antibody
    • AI661148 antibody
    • autonomic nervous system and neural crest derivatives-expressed protein 2 antibody
    • Basic helix loop helix transcription factor HAND2 antibody
    • bHLHa26 antibody
    • Class A basic helix-loop-helix protein 26 antibody
    • Deciduum antibody
    • Deciduum heart autonomic nervous system and neural crest derivatives expressed protein 2 antibody
    • DHAND 2 antibody
    • dHAND antibody
    • DHAND2 antibody
    • Ehand 2 antibody
    • Ehand2 antibody
    • FLJ16260 antibody
    • HAND 2 antibody
    • Hand2 antibody
    • HAND2_HUMAN antibody
    • Heart and neural crest derivatives expressed 2 antibody
    • Heart and neural crest derivatives expressed protein 2 antibody
    • heart and neural crest derivatives expressed transcript 2 antibody
    • heart antibody
    • Heart- and neural crest derivatives-expressed protein 2 antibody
    • Hed antibody
    • Highly similar to dHAND M musculus antibody
    • MGC125303 antibody
    • MGC125304 antibody
    • Th 2 antibody
    • Th2 antibody
    • Thing 2 antibody
    • Thing2 antibody
    see all

Anti-HAND2 antibody - Carboxyterminal end images

  • ab60037 at 1/50 dilution staining HAND2 in Human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. A peroxidase-conjugated secondary antibody was used, followed by AEC staining.

References for Anti-HAND2 antibody - Carboxyterminal end (ab60037)

ab60037 has not yet been referenced specifically in any publications.

Product Wall

Thank you for calling us and for alerting us to the problem you are experiencing with the anti-HAND2 antibody (ab60037). We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

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We have 3 antibodies available in Abcam catalogue; the numbers are ab10131, ab56590 and ab60037. Please choose any one of these for testing.

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Dogfish/Catshark Tissue sections (Stage 28 embryos. Level of posterior pectoral fin)
Specification
Stage 28 embryos. Level of posterior pectoral fin
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Nov 10 2011

Thank you for getting back to me and for providing some further information. To our knowledge, ab60037 has not been tested in catshark - only in human. The immunogenic sequence of ab60037 shares 93% homology with catshark and it is very surprisi...

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Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with our product. This antibody has only been tested in human only and ...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Cartilage)
Specification
Cartilage
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 19°C
Username

Abcam user community

Verified customer

Submitted Jan 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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