Reacts with nucleocapsid proteins of Puumala hantaviruses: Vranica, Sotkamo and Kazan and reacts with hantavirus-infected cells. It reacts with nucleocapsid proteins of Andes, Sin Nombre and Seoul hantaviruses. It does not cross-react with hamster polyomavirus major capsid protein VP1
Not yet tested in other species.
Recombinant fragment corresponding to Hantavirus nucleocapsid protein.
The epitope recognized by this antibody is localized at N-terminus, between amino acids 1 and 45 of the hantavirus nucleocapsid protein.
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR148555-9 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000.
1/1000 - 1/5000.
Use at an assay dependent dilution. PubMed: 18687679
The members of the Hantavirus genus of the family Bunyaviridae are spherical, enveloped viruses containing tripartite negative-sense RNA as their genome. The three genomic RNA segments, designated L, M, and S, encode an RNA-dependent RNA polymerase, envelope glycoproteins (G1 and G2), and nucleocapsid (N) protein, respectively. Hantavirus infections can cause two serious and often fatal human diseases, hemorrhagic fever with renal syndrome and hantaviral pulmonary syndrome, characterized by lung damage and cardiac dysfunction. Humans are infected with hantaviruses from rodent reservoirs that are persistently infected without signs of disease.
Western blot - Anti-Hantavirus nucleocapsid protein antibody [5E11] (ab34757)
ab34757 at 1 µg/mL used in Western blot of recombinant nucleocapsid proteins.
Mertens M et al. Phylogenetic analysis of Puumala virus subtype Bavaria, characterization and diagnostic use of its recombinant nucleocapsid protein. Virus Genes43:177-91 (2011).
Read more (PubMed: 21598005) »
Wang Y et al. NMR structure of the N-terminal coiled coil domain of the Andes hantavirus nucleocapsid protein. J Biol Chem283:28297-304 (2008).
Read more (PubMed: 18687679) »