Anti-Hantavirus nucleocapsid protein antibody [5E11] (ab34757)

Overview

  • Product name
    Anti-Hantavirus nucleocapsid protein antibody [5E11]
    See all Hantavirus nucleocapsid protein primary antibodies
  • Description
    Mouse monoclonal [5E11] to Hantavirus nucleocapsid protein
  • Host species
    Mouse
  • Tested applications
    Suitable for: Indirect ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with nucleocapsid proteins of Puumala hantaviruses: Vranica, Sotkamo and Kazan and reacts with hantavirus-infected cells. It reacts with nucleocapsid proteins of Andes, Sin Nombre and Seoul hantaviruses. It does not cross-react with hamster polyomavirus major capsid protein VP1 Not yet tested in other species.
  • Immunogen

    Recombinant fragment corresponding to Hantavirus nucleocapsid protein.

  • Epitope
    The epitope recognized by this antibody is localized at N-terminus, between amino acids 1 and 45 of the hantavirus nucleocapsid protein.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR148555-9 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    5E11
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA 1/1000 - 1/10000.
WB 1/1000 - 1/5000.
ICC/IF Use at an assay dependent dilution. PubMed: 18687679

Target

  • Relevance
    The members of the Hantavirus genus of the family Bunyaviridae are spherical, enveloped viruses containing tripartite negative-sense RNA as their genome. The three genomic RNA segments, designated L, M, and S, encode an RNA-dependent RNA polymerase, envelope glycoproteins (G1 and G2), and nucleocapsid (N) protein, respectively. Hantavirus infections can cause two serious and often fatal human diseases, hemorrhagic fever with renal syndrome and hantaviral pulmonary syndrome, characterized by lung damage and cardiac dysfunction. Humans are infected with hantaviruses from rodent reservoirs that are persistently infected without signs of disease.

Images

  • ab34757 at 1 µg/mL used in Western blot of recombinant nucleocapsid proteins.

    Lane 1 Sotkamo NP

    Lanes 2 & 3 Vranica NP

    Lane 4 Kazan NP

References

This product has been referenced in:
  • Mertens M  et al. Phylogenetic analysis of Puumala virus subtype Bavaria, characterization and diagnostic use of its recombinant nucleocapsid protein. Virus Genes 43:177-91 (2011). Read more (PubMed: 21598005) »
  • Wang Y  et al. NMR structure of the N-terminal coiled coil domain of the Andes hantavirus nucleocapsid protein. J Biol Chem 283:28297-304 (2008). ICC/IF . Read more (PubMed: 18687679) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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