Anti-Hantavirus nucleocapsid protein antibody [5E11] (ab34759)

Overview

  • Product name
    Anti-Hantavirus nucleocapsid protein antibody [5E11]
    See all Hantavirus nucleocapsid protein primary antibodies
  • Description
    Mouse monoclonal [5E11] to Hantavirus nucleocapsid protein
  • Host species
    Mouse
  • Specificity
    This antibody reacts with nucleocapsid proteins of Puumala hantaviruses: Vranica, Sotkamo and Kazan and reacts with hantavirus-infected cells. It cross-reacts with nucleocapsid proteins of Andes, Sin Nombre and Seoul hantaviruses. It does not cross-react with hamster polyomavirus major capsid protein VP1, nor with nucleocapsid proteins of measles and mumps viruses.
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Reacts with hantavirus. Not yet tested in other species.
  • Immunogen

    Recombinant fragment -recombinant chimeric protein harbouring N-terminal segment (1-120 aa) of Puumala hantavirus (strain Vranica-Hällnäs) nucleocapsid protein, expressed in yeast S.cerevisiae.

  • Epitope
    The epitope recognized by this antibody is localized at the N-terminus, between amino acids 1 and 45 of hantavirus nucleocapsid protein.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    5E11
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1 - 1/20.

Target

  • Relevance
    The members of the Hantavirus genus of the family Bunyaviridae are spherical, enveloped viruses containing tripartite negative-sense RNA as their genome. The three genomic RNA segments, designated L, M, and S, encode an RNA-dependent RNA polymerase, envelope glycoproteins (G1 and G2), and nucleocapsid (N) protein, respectively. Hantavirus infections can cause two serious and often fatal human diseases, hemorrhagic fever with renal syndrome and hantaviral pulmonary syndrome, characterized by lung damage and cardiac dysfunction. Humans are infected with hantaviruses from rodent reservoirs that are persistently infected without signs of disease.

References

This product has been referenced in:
  • Perelygina L  et al. Persistent infection of human fetal endothelial cells with rubella virus. PLoS One 8:e73014 (2013). WB ; Human . Read more (PubMed: 23940821) »
  • Schlegel M  et al. Novel serological tools for detection of Thottapalayam virus, a Soricomorpha-borne hantavirus. Arch Virol 157:2179-87 (2012). Read more (PubMed: 22828778) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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