Overview

  • Product nameAnti-Hantavirus nucleocapsid protein antibody
    See all Hantavirus nucleocapsid protein primary antibodies
  • Description
    Mouse monoclonal to Hantavirus nucleocapsid protein
  • SpecificityDoes not cross-react with nucleocapsid proteins of measles and mumps viruses.
  • Tested applicationsSuitable for: Indirect ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with nucleocapsid proteins of Puumala hantaviruses: Vranica, Sotkamo and Kazan and reacts with hantavirus-infected cells. It reacts with nucleocapsid proteins of Andes, Sin Nombre and Seoul hantaviruses. It does not cross-react with hamster polyomavirus major capsid protein VP1 Not yet tested in other species.
  • Immunogen

    Recombinant fragment - recombinant chimeric protein harbouring N-terminal segment (1-120 aa) of Puumala hantavirus (strain Vranica-Hällnäs) nucleocapsid protein, expressed in yeast S.cerevisiae.

  • EpitopeThe epitope recognized by this antibody is localized at N-terminus, between amino acids 1 and 45 of the hantavirus nucleocapsid protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • MyelomaSp2/0
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA 1/1000 - 1/10000.
WB 1/1000 - 1/5000.
ICC/IF Use at an assay dependent dilution. PubMed: 18687679

Target

  • RelevanceThe members of the Hantavirus genus of the family Bunyaviridae are spherical, enveloped viruses containing tripartite negative-sense RNA as their genome. The three genomic RNA segments, designated L, M, and S, encode an RNA-dependent RNA polymerase, envelope glycoproteins (G1 and G2), and nucleocapsid (N) protein, respectively. Hantavirus infections can cause two serious and often fatal human diseases, hemorrhagic fever with renal syndrome and hantaviral pulmonary syndrome, characterized by lung damage and cardiac dysfunction. Humans are infected with hantaviruses from rodent reservoirs that are persistently infected without signs of disease.

Anti-Hantavirus nucleocapsid protein antibody images

  • ab34757 at 1µg/ml used in Western blot of recombinant nucleocapsid proteins.

    Lane 1 Sotkamo NP

    Lanes 2 & 3 Vranica NP

    Lane 4 Kazan NP

References for Anti-Hantavirus nucleocapsid protein antibody (ab34757)

This product has been referenced in:
  • Wang Y  et al. NMR structure of the N-terminal coiled coil domain of the Andes hantavirus nucleocapsid protein. J Biol Chem 283:28297-304 (2008). ICC/IF . Read more (PubMed: 18687679) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"