Reacts with nucleocapsid proteins of Puumala hantaviruses: Vranica, Sotkamo and Kazan and reacts with hantavirus-infected cells. It reacts with nucleocapsid proteins of Andes, Sin Nombre and Seoul hantaviruses. It does not cross-react with hamster polyomavirus major capsid protein VP1
Not yet tested in other species.
Recombinant fragment - recombinant chimeric protein harbouring N-terminal segment (1-120 aa) of Puumala hantavirus (strain Vranica-Hällnäs) nucleocapsid protein, expressed in yeast S.cerevisiae.
EpitopeThe epitope recognized by this antibody is localized at N-terminus, between amino acids 1 and 45 of the hantavirus nucleocapsid protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000.
1/1000 - 1/5000.
Use at an assay dependent dilution. PubMed: 18687679
RelevanceThe members of the Hantavirus genus of the family Bunyaviridae are spherical, enveloped viruses containing tripartite negative-sense RNA as their genome. The three genomic RNA segments, designated L, M, and S, encode an RNA-dependent RNA polymerase, envelope glycoproteins (G1 and G2), and nucleocapsid (N) protein, respectively. Hantavirus infections can cause two serious and often fatal human diseases, hemorrhagic fever with renal syndrome and hantaviral pulmonary syndrome, characterized by lung damage and cardiac dysfunction. Humans are infected with hantaviruses from rodent reservoirs that are persistently infected without signs of disease.
Anti-Hantavirus nucleocapsid protein antibody images
Western blot - Hantavirus nucleocapsid protein antibody (ab34757)
ab34757 at 1µg/ml used in Western blot of recombinant nucleocapsid proteins.
Lane 1 Sotkamo NP
Lanes 2 & 3 Vranica NP
Lane 4 Kazan NP
References for Anti-Hantavirus nucleocapsid protein antibody (ab34757)
This product has been referenced in:
Wang Y et al. NMR structure of the N-terminal coiled coil domain of the Andes hantavirus nucleocapsid protein. J Biol Chem283:28297-304 (2008).
Read more (PubMed: 18687679) »