Overview

  • Product nameAnti-HAPLN1 antibody
    See all HAPLN1 primary antibodies
  • Description
    Rabbit polyclonal to HAPLN1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 17-46 of Human HAPLN1.

  • Positive control
    • MCF-7, Jurkat and HepG2 cell lysates; Human prostate carcinoma

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab103455 is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 40 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionStabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix.
  • Tissue specificityWidely expressed. Weakly expressed in the brain.
  • Sequence similaritiesBelongs to the HAPLN family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    Contains 2 Link domains.
  • Cellular localizationSecreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cartilage link protein antibody
    • cartilage linking protein 1 antibody
    • Cartilage-link protein antibody
    • Cartilage-linking protein 1 antibody
    • CLP antibody
    • CRTL1 antibody
    • Crtl1l antibody
    • HAPLN1 antibody
    • HPLN1_HUMAN antibody
    • Hyaluronan and proteoglycan link protein 1 antibody
    • Hyaluronan and proteoglycan link protein 1 precursor antibody
    • LP antibody
    • Proteoglycan link protein antibody
    see all

Anti-HAPLN1 antibody images

  • All lanes : Anti-HAPLN1 antibody (ab103455) at 1/100 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 40 kDa
  • ab103455, at 1/50 dilution, staining HAPLN1 in formalin-fixed, paraffin-embedded Human prostate carcinoma by Immunohistochemistry using a peroxidase conjugated secondary antibody and DAB staining.

References for Anti-HAPLN1 antibody (ab103455)

This product has been referenced in:
  • Zheng J  et al. Abnormal expression of chondroitin sulphate N-acetylgalactosaminyltransferase 1 and Hapln-1 in cartilage with Kashin-Beck disease and primary osteoarthritis. Int Orthop 37:2051-9 (2013). WB, IHC-P ; Human . Read more (PubMed: 23748413) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"