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Storage instructions Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Storage buffer Preservative: 0.09% Sodium Azide
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab103455 is purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 40 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Function Stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix.
Tissue specificity Widely expressed. Weakly expressed in the brain.
Sequence similarities Belongs to the HAPLN family. Contains 1 Ig-like V-type (immunoglobulin-like) domain. Contains 2 Link domains.
Cellular localization Secreted, extracellular space, extracellular matrix.
Information by UniProt
Cartilage link protein antibody
cartilage linking protein 1 antibody
Cartilage-link protein antibody
Anti-HAPLN1 antibody images
Western blot - HAPLN1 antibody (ab103455)
All lanes : Anti-HAPLN1 antibody (ab103455) at 1/100 dilution Lane 1 : MCF-7 cell lysate Lane 2 : Jurkat cell lysate Lane 3 : HepG2 cell lysate Lysates/proteins at 35 µg per lane. Predicted band size : 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HAPLN1 antibody (ab103455)
ab103455, at 1/50 dilution, staining HAPLN1 in formalin-fixed, paraffin-embedded Human prostate carcinoma by Immunohistochemistry using a peroxidase conjugated secondary antibody and DAB staining.
References for Anti-HAPLN1 antibody (ab103455)
This product has been referenced in:
et al. Abnormal expression of chondroitin sulphate N-acetylgalactosaminyltransferase 1 and Hapln-1 in cartilage with Kashin-Beck disease and primary osteoarthritis. Int Orthop 37:2051-9 (2013).
Read more (PubMed: 23748413) »
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for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"