Recombinant
RabMAb

Anti-HAPLN1 antibody [EPR6338] (ab181997)

Overview

  • Product name
    Anti-HAPLN1 antibody [EPR6338]
    See all HAPLN1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6338] to HAPLN1
  • Tested applications
    Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: P10915

  • Positive control
    • Human spleen, MCF7 and Caco2 lysates; Human transitional cell carcinoma of bladder tissue; MCF7 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR6338
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/2000.
WB 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa).
IHC-P 1/50.
ICC/IF 1/250.

Target

  • Function
    Stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix.
  • Tissue specificity
    Widely expressed. Weakly expressed in the brain.
  • Sequence similarities
    Belongs to the HAPLN family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    Contains 2 Link domains.
  • Cellular localization
    Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cartilage link protein antibody
    • cartilage linking protein 1 antibody
    • Cartilage-link protein antibody
    • Cartilage-linking protein 1 antibody
    • CLP antibody
    • CRTL1 antibody
    • Crtl1l antibody
    • HAPLN1 antibody
    • HPLN1_HUMAN antibody
    • Hyaluronan and proteoglycan link protein 1 antibody
    • Hyaluronan and proteoglycan link protein 1 precursor antibody
    • LP antibody
    • Proteoglycan link protein antibody
    see all

Images

  • All lanes : Anti-HAPLN1 antibody [EPR6338] (ab181997) at 1/1000 dilution

    Lane 1 : MCF7 lysate
    Lane 2 : Caco2 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L)-HRP at 1/1000 dilution

    Predicted band size : 40 kDa
  • Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling HAPLN1 with ab181997 at 1/50 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Immunofluorescent analysis of MCF7 cells (paraformaldehyde-fixed, 4%) labeling HAPLN1 with ab181997 at 1/250 dilution, followed by Goat anti rabbit IgG(Dylight 488) at 1/200 dilution and counter-stained with DAPI (blue).

  • Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling HAPLN1 with purified ab181997 at 1/2000 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

  • Anti-HAPLN1 antibody [EPR6338] (ab181997) at 1/1000 dilution + Human spleen lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/1000 dilution

    Predicted band size : 40 kDa

References

ab181997 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Hippocampal)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Hippocampal
Blocking step
Licor fluorescent blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 23°C
Username

Maral Tajerian

Verified customer

Submitted Jul 20 2016

Application
Flow Cytometry
Sample
Human Cell (chondrocyte)
Permeabilization
Yes - BD Perm Wash Buffer
Gating Strategy
live cells of median size
Specification
chondrocyte
Preparation
Cell harvesting/tissue preparation method: fixed and permeabilized cells
Sample buffer: BD Permeabilization Wash Buffer
Fixation
BD Fix Perm Buffer
Username

Natalie Woolery

Verified customer

Submitted Aug 03 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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