Anti-Haploid sperm cell-specific antigen antibody [TRA54] (ab92286)

Overview

  • Product name
    Anti-Haploid sperm cell-specific antigen antibody [TRA54]
  • Description
    Rat monoclonal [TRA54] to Haploid sperm cell-specific antigen
  • Tested applications
    Suitable for: WB, Electron Microscopy, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Adult Mouse testis.

  • Positive control
    • Mouse testis lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: None
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purification notes
    ab92286 was purified from the serum-free culture medium of the hybridoma clone TRA54 by proprietary chromatography processes under mild conditions.
  • Clonality
    Monoclonal
  • Clone number
    TRA54
  • Isotype
    IgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab92286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml.
Electron Microscopy Use at an assay dependent dilution. PubMed: 12857863
IHC-P Use at an assay dependent dilution.

Target

  • Relevance
    Many transcripts for proteins specifically expressed in haploid germ cells are synthesized in the spermatogenesis. A monoclonal antibody TRA54 recognizes specific organellaes in germ cell cytoplasm from spermatocytes to spermatids.

Images

  • All lanes : Anti-Haploid sperm cell-specific antigen antibody [TRA54] (ab92286) at 1/5000 dilution

    Lane 1 : Mouse brain cell lysate
    Lane 2 : Mouse Heart cell lysate
    Lane 3 : Mouse Intestine cell lysate
    Lane 4 : Mouse Kidney cell lysate
    Lane 5 : Mouse Liver cell lysate
    Lane 6 : Mouse Muscle cell lysate
    Lane 7 : Mouse Ovary cell lysate
    Lane 8 : Mouse Skin cell lysate
    Lane 9 : Mouse Spleen cell lysate
    Lane 10 : Mouse Testis cell lysate

    Lysates/proteins at 100 µg per lane.


    Observed band size : 190,85,>200 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Arroteia KF  et al. Albumin is synthesized in epididymis and aggregates in a high molecular mass glycoprotein complex involved in sperm-egg fertilization. PLoS One 9:e103566 (2014). WB ; Mouse . Read more (PubMed: 25084016) »
  • Arrotéia KF  et al. Identification and characterization of an antigen recognized by monoclonal antibody TRA 54 in mouse epididymis and vas deferens. J Androl 25:914-21 (2004). Read more (PubMed: 15477363) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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