The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
Use at an assay dependent dilution. PubMed: 12857863
Use at an assay dependent dilution.
Many transcripts for proteins specifically expressed in haploid germ cells are synthesized in the spermatogenesis. A monoclonal antibody TRA54 recognizes specific organellaes in germ cell cytoplasm from spermatocytes to spermatids.
References for Anti-Haploid sperm cell-specific antigen antibody [TRA54] (ab92286)
This product has been referenced in:
Arroteia KF et al. Albumin is synthesized in epididymis and aggregates in a high molecular mass glycoprotein complex involved in sperm-egg fertilization. PLoS One9:e103566 (2014).
Read more (PubMed: 25084016) »
Arrotéia KF et al. Identification and characterization of an antigen recognized by monoclonal antibody TRA 54 in mouse epididymis and vas deferens. J Androl25:914-21 (2004).
Read more (PubMed: 15477363) »