SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti Rabbit Serum. Analysis by SDS PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG.
Purification notesPurification of pooled rabbit sera was by Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification. The eluate was
dialyzed against PBS with sodium azide, concentrated by Amicon ultrafiltration, 0.2µ filtered, aseptically filled, and stored at 2°
to 8° C.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/3000 - 1/12000. with TMB detection substrate.
FunctionAs a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway. Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
Tissue specificityExpressed by the liver and secreted in plasma.
Involvement in diseaseAnhaptoglobinemia
Sequence similaritiesBelongs to the peptidase S1 family. Contains 1 peptidase S1 domain. Contains 2 Sushi (CCP/SCR) domains.