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Anti-Haptoglobin antibody (ab85846)

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Overview

  • Product nameAnti-Haptoglobin antibodySee all Haptoglobin primary antibodies ...
  • Description
    Rabbit polyclonal to Haptoglobin
  • Tested applicationsWB, ELISA, IHC-P, Sandwich ELISA more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human Haptoglobin

  • Positive controlHuman liver tissue

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesab85846 recognizes Human Haptoglobin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab85846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/2000 - 1/10000. Predicted molecular weight: 45 kDa.
ELISA ELISA: 1/3000 - 1/12000.
IHC-P IHC-P: Use a concentration of 2.5 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA sELISA: Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse polyclonal to Haptoglobin (ab52652). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse polyclonal to Haptoglobin (ab52652) as Capture.

Target

  • FunctionAs a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
    Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
  • Tissue specificityExpressed by the liver and secreted in plasma.
  • Involvement in diseaseAnhaptoglobinemia (AHP) [MIM:614081]: A condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin. Congenital haptoglobin deficiency is a risk factor for anaphylactic non-hemolytic transfusion reactions. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P00738 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Binding peptide antibodyBP antibodyHaptoglobin alpha chain antibody
        Haptoglobin alpha(1S) beta antibodyHaptoglobin alpha(2FS) beta antibodyHaptoglobin beta chain antibodyHaptoglobin, alpha polypeptide antibodyHaptoglobin, beta polypeptide antibodyHP antibodyHP2 ALPHA2 antibodyHP2ALPHA2 antibodyHPA1S antibodyHPT antibodyHPT_HUMAN antibodyMGC111141 antibodyZonulin antibody
      see all

    Anti-Haptoglobin antibody images

    Protocols

    References for Anti-Haptoglobin antibody (ab85846)

    ab85846 has not yet been referenced specifically in any publications.

    Publishing research using ab85846 ? Please let us know so that we can cite the reference in this datasheet

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    Thank you for the note. We will order these antibodies for haptoglobin and free hemoglobin. Wonder if they are good to use for human blood. Do you have standard to control the reaction.

    These two products will be suitable standards:

    Hemoglobin - ab77858 (purified from Human erythrocytes)
    Haptoglobin - ab77872 (purified from Human plasma)

    Please contact us if you have additional questions.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"