FunctionAs a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway. Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
Tissue specificityExpressed by the liver and secreted in plasma.
Involvement in diseaseAnhaptoglobinemia
Sequence similaritiesBelongs to the peptidase S1 family. Contains 1 peptidase S1 domain. Contains 2 Sushi (CCP/SCR) domains.
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, using ab97976 at 1/200 dilution. Lower image is merged with DNA probe.
Western blot - Haptoglobin antibody (ab97976)
Anti-Haptoglobin antibody (ab97976) at 1/5000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size : 45 kDa
References for Anti-Haptoglobin antibody (ab97976)
This product has been referenced in:
Perdijk O et al. Haptoglobin phenotype prevalence and cytokine profiles during Plasmodium falciparum infection in Dogon and Fulani ethnic groups living in Mali. Malar J12:432 (2013).
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