Anti-Haptoglobin antibody [HG-36] (ab13429)
Key features and details
- Mouse monoclonal [HG-36] to Haptoglobin
- Suitable for: ELISA, Dot blot, ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-Haptoglobin antibody [HG-36]
See all Haptoglobin primary antibodies -
Description
Mouse monoclonal [HG-36] to Haptoglobin -
Host species
Mouse -
Tested applications
Suitable for: ELISA, Dot blot, ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (Purified haptoglobin types 1-1, 2-1, and 2-2 from pooled human plasma).
-
General notes
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.098% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
-
Purity
Proprietary Purification -
Purification notes
Purified from hybridoma cell culture -
Clonality
Monoclonal -
Clone number
HG-36 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
Dot blot |
Use at an assay dependent concentration.
|
|
ICC/IF | (2) |
Use at an assay dependent concentration.
|
WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
|
IHC-P | (4) |
Use at an assay dependent concentration.
See Abreview. |
Notes |
---|
ELISA
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 45 kDa. |
IHC-P
Use at an assay dependent concentration. See Abreview. |
Target
-
Function
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Anhaptoglobinemia -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 3240 Human
- Omim: 140100 Human
- SwissProt: P00738 Human
- Unigene: 513711 Human
- Unigene: 702099 Human
-
Alternative names
- Binding peptide antibody
- BP antibody
- Haptoglobin alpha chain antibody
see all
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (18)
ab13429 has been referenced in 18 publications.
- Gunzer S et al. Hypertransaminasemia and liver fibrosis associated with haptoglobin retention and anhaptoglobinemia in a paediatric patient. Liver Int 41:2427-2432 (2021). PubMed: 34358398
- Calvo E et al. Why Does COVID-19 Affect Patients with Spinal Cord Injury Milder? A Case-Control Study: Results from Two Observational Cohorts. J Pers Med 10:N/A (2020). PubMed: 33096722
- Cao J et al. Identification of an activation-related protein in B cells in the ABO incompatible condition. Exp Ther Med 19:741-747 (2020). PubMed: 31897108
- Arya R et al. Serum Small Extracellular Vesicles Proteome of Tuberculosis Patients Demonstrated Deregulated Immune Response. Proteomics Clin Appl 14:e1900062 (2020). PubMed: 31532894
- Gallo JRB et al. Skin Protein Profile after Major Weight Loss and Its Role in Body Contouring Surgery. Plast Reconstr Surg Glob Open 7:e2339 (2019). PubMed: 31592376