Overview

  • Product name
    Anti-Haptoglobin antibody [HG-36]
    See all Haptoglobin primary antibodies
  • Description
    Mouse monoclonal [HG-36] to Haptoglobin
  • Specificity
    This antibody reacts with the three types of human haptoglobin (types 1-1, 2-1, and 2-2). In an ELISA, the antibody recognizes human haptoglobin as the purified antigen, in human serum, when complexed with human hemoglobin or captured by antibodies. In a competitive ELISA it does not cross react with human IgG, transferrin, C-reactive protein, alpha 1 acid glycoprotein, albumin or hemoglobin, nor with bovine, goat or sheep serum. The antibody reacts against native and denatured (non-reduced) human haptoglobin in an immunoblot or dot blot but fails to recognize the different haptoglobin types under reducing conditions.
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICC/IF, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (Purified haptoglobin types 1-1, 2-1, and 2-2 from pooled human plasma).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: Ascites
  • Concentration information loading...
  • Purity
    Ascites
  • Clonality
    Monoclonal
  • Clone number
    HG-36
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab13429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Use under non reducing condition.
ELISA 1/4000.
IHC-P Use at an assay dependent concentration.

See Abreview.

ICC/IF Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.

Target

  • Function
    As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
    Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
  • Tissue specificity
    Expressed by the liver and secreted in plasma.
  • Involvement in disease
    Anhaptoglobinemia
  • Sequence similarities
    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Binding peptide antibody
    • BP antibody
    • Haptoglobin alpha chain antibody
    • Haptoglobin alpha(1S) beta antibody
    • Haptoglobin alpha(2FS) beta antibody
    • Haptoglobin beta chain antibody
    • Haptoglobin, alpha polypeptide antibody
    • Haptoglobin, beta polypeptide antibody
    • HP antibody
    • HP2 ALPHA2 antibody
    • HP2ALPHA2 antibody
    • HPA1S antibody
    • HPT antibody
    • HPT_HUMAN antibody
    • MGC111141 antibody
    • Zonulin antibody
    see all

Anti-Haptoglobin antibody [HG-36] images

  • This image is courtesy of an anonymous Abreview.

    ab13429 used in Western Blot.
    Tissue lysate was obtained from HOPE-fixed paraffin-embedded blocks of human lung, lung cancer and liver tissue, loaded at 30µg/ml. ab13429 used at a 1/100 dilution. Secondary used was an alkaline phosphatase goat anti-mouse polyclonal used at a 1/10000 dilution.

    See Abreview

  • ab13429 staining Haptoglobin in human bronchoalveolar lavage cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in HOPE, blocked for 5 minutes at 25°C, then incubated with ab13429 at a 1/100 for 1 hour at 25°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal used at a 1/200 dilution. Counterstained with DAPI.

    See Abreview

  • ab13429 staining Haptoglobin in human lung cancer tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed with HOPE, blocked for 5 minutes at 25°C, then incubated with ab13429 at a 1/100 dilution for 1 hour at 25°C. The secondary used was an undiluted HRP conjugated goat polyclonal.

    See Abreview

References for Anti-Haptoglobin antibody [HG-36] (ab13429)

This product has been referenced in:
  • Yang Q  et al. iTRAQ-Based Proteomics Investigation of Aqueous Humor from Patients with Coats' Disease. PLoS One 11:e0158611 (2016). WB . Read more (PubMed: 27416065) »
  • Tomazic PV  et al. Nasal mucus proteomic changes reflect altered immune responses and epithelial permeability in patients with allergic rhinitis. J Allergy Clin Immunol N/A:N/A (2013). WB ; Human . Read more (PubMed: 24290289) »

See all 5 Publications for this product

Product Wall

Thank you for your email.

Generally antibodies are stable for 1 year at 4C and for 10 years at -20C or -80C. We however recommend storing the antibodies at 4C for short term (1-2 weeks) because some antibodies lose activity if they kept at 4...

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Thank you for your response and your comment.
The on-line document contains general information/guidelines for our antibodies for different purifications. Each product has specific storage instructions which can be found on the public website (on-...

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Thank you for your enquiry and your interest in our products.
This antibody is ascitic fluid and the storage buffer contains 15mM sodium azide. You could use PBS for diluting the antibody. We have some tips/recommendations at this site, you may fi...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Lung, lung cancer and liver)
Loading amount
30 µg
Specification
Lung, lung cancer and liver
Gel Running Conditions
Reduced Denaturing (4%stacking, 12%resolving)
Blocking step
DIG Wash & Block Buffer, Roche as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 08 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung, lung cancer)
Specification
Lung, lung cancer
Fixative
HOPE (Hepes-glutamic acid buffer mediated Organic solvent Protection Effect Olert et al.,2001 Pathol Res Pract)
Antigen retrieval step
None
Permeabilization
No
Blocking step
Zytomed Systems Blocking Solution as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 03 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human bronchoalveolar lavage cells mainly macroph)
Specification
human bronchoalveolar lavage cells mainly macroph
Fixative
HOPE (Hepes-glutamic acid buffer mediated Organic solvent Protection Effect Olert et al.,2001 Pathol Res Pract)
Permeabilization
No
Blocking step
Zytomed Systems Blocking Solution as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 03 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (liver,spleen)
Specification
liver,spleen
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
H²O²/Methanol as blocking agent for 15 minute(s) · Concentration: 1%
Username

Abcam user community

Verified customer

Submitted Dec 12 2006

Thank you for your enquiry. We have not epitope mapped this antibody. The antibody recognizes the 3 haptoglobin types in ELISA, dot-blot and immunoblotting. The 3 haptoglobin types have an identical beta-chain. It is likely that the antibody reco...

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Thank you for your enquiry. For ab13429, the antibody recognizes human haptoglobin as the purified antigen, in human serum, when complexed with human hemoglobin or captured by antibodies. In a competitive ELISA it does not cross react with human IgG, t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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