Overview

  • Product nameHaptoglobin Human ELISA KitSee all Haptoglobin kits ...
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.1%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine
  • Assay typeSandwich (quantitative)
  • Sensitivity
    > 3.1 ng/ml
  • Range
    3.125 ng/ml - 200 ng/ml
  • Recovery

    98.5 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Haptoglobin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Haptoglobin concentrations in Human urine, saliva, milk and cell culture supernatants.

    A Haptoglobin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Haptoglobin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Haptoglobin captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionHaptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes.
    Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
  • Tissue specificityExpressed by the liver and secreted in plasma.
  • Involvement in diseaseDefects in HP are the cause of anhaptoglobinemia (AHP) [MIM:614081]. AHP is a condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • binding peptide
    • BP
    • haptoglobin alpha 1S beta
    • haptoglobin alpha 2FS beta
    • haptoglobin alpha polypeptide
    • Haptoglobin beta chain
    • haptoglobin beta polypeptide
    • HP
    • HP2ALPHA2
    • HPA1S
    • HPT_HUMAN
    • Zonulin
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Haptoglobin Human ELISA Kit images

  • Representative Standard Curve using ab108858.

Protocols

References for Haptoglobin Human ELISA Kit (ab108858)

ab108858 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us and your interest in our products. I am sorry for the delay in getting back to you.
The Haptoglobulin Human ELISA Kits (ab108856 and ab108858) can be used in order to quantify the level of haptoglobulin in various biolo...

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Te pido disculpas por la espera y agradezco tu paciencia.

Me confirman mis compañeros del laboratorio que ambos kits (ab108856 y ab108858) detectan la subunidad beta de la proteína. Además, si la sensibilidad del kit ab10...

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Muchas gracias por contactarnos.

El kit anticuerpo contenido en el kit ab108858 reconoce la sub unidad beta de la Haptoglobina.

Si necesitas más información, no dudes en contactarnos de nuevo.

Thank you for contacting us and sorry for the delay in getting back to you. Your customer is correct in thinking that the Haptoglobin and Haptoglobulin refer to the same protein. I am sorry that our datasheets have caused confusion. I will be looki...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"