Anti-Haptoglobulin beta antibody (ab126842)

Overview

  • Product name
    Anti-Haptoglobulin beta antibody
    See all Haptoglobulin beta primary antibodies
  • Description
    Goat polyclonal to Haptoglobulin beta
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human, Pig
    Predicted to work with: Dog, Chimpanzee, Baboon, Macaque monkey, Gorilla, Common marmoset, Orangutan, Bat
  • Immunogen

    Synthetic peptide:

    C-STVPEKKTPKSP

    (with a Cysteine residue linker), corresponding to internal sequence amino acids 316-327 of Human Haptoglobulin beta (NP_005134.1; NP_001119574.1).

  • Positive control
    • Human Liver lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 45 kDa.

Target

Images

  • Anti-Haptoglobulin beta antibody (ab126842) at 0.3 µg/ml + pig liver tissue lysate

    Predicted band size : 45 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Anti-Haptoglobulin beta antibody (ab126842) at 0.1 µg/ml + Human Liver lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 45 kDa
  • Lane 1 :
    Lanes 2 - 3 : Anti-Haptoglobulin beta antibody (ab126842) at 1/20 dilution

    Lane 1 : Marker
    Lane 2 : Pig endometrial tissue lysate - whole at 30 µg
    Lane 3 : Pig endometrial tissue lysate - whole at 30 µg

    Secondary
    Lanes 2 - 3 : Alkaline phosphatase rabbit anti-goat IgG polyclonal at 1/20000 dilution


    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 27 kDa (possible non-specific binding),52 kDa (possible non-specific binding).

    Exposure time : 24 minutes

    This image is courtesy of an anonymous Abreview

    52kDa band is from the secondary antibody.

    See Abreview

References

ab126842 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12.5%)
Sample
Pig Tissue lysate - whole (Porcine endometrial tissue lysate)
Specification
Porcine endometrial tissue lysate
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

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Verified customer

Submitted May 15 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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