The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000.
1/500 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
Tissue specificityBrain, heart, liver and kidney.
Involvement in diseaseDefects in HARS are a cause of Usher syndrome type 3B (USH3B) [MIM:614504]. USH3B is a syndrome characterized by progressive vision and hearing loss during early childhood. Some patients have the so-called 'Charles Bonnet syndrome,' involving decreased visual acuity and vivid visual hallucinations. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa with sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH3 is characterized by postlingual, progressive hearing loss, variable vestibular dysfunction, and onset of retinitis pigmentosa symptoms, including nyctalopia, constriction of the visual fields, and loss of central visual acuity, usually by the second decade of life.
Sequence similaritiesBelongs to the class-II aminoacyl-tRNA synthetase family. Contains 1 WHEP-TRS domain.
ab65014 at 1/500 - 1/1000 dilution staining HARS in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
Western blot - HARS antibody (ab65014)
All lanes : Anti-HARS antibody (ab65014) at 1/500 dilution
Lane 1 : HeLa cell extract Lane 2 : HeLa cell extract with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 56 kDa Observed band size : 56 kDa Additional bands at : 20 kDa,37 kDa,40 kDa. We are unsure as to the identity of these extra bands.
References for Anti-HARS antibody (ab65014)
This product has been referenced in:
Sciorati C et al. Required role of apoptotic myogenic precursors and toll-like receptor stimulation for the establishment of autoimmune myositis in experimental murine models. Arthritis Rheumatol67:809-22 (2015).
Read more (PubMed: 25504878) »