• Product nameAnti-HARS antibody
    See all HARS primary antibodies
  • Description
    Rabbit polyclonal to HARS
  • Tested applicationsSuitable for: ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from C-terminal of human HARS.

  • Positive control
    • HeLa cells. HepG2 cells.



Our Abpromise guarantee covers the use of ab65014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).


  • Tissue specificityBrain, heart, liver and kidney.
  • Involvement in diseaseDefects in HARS are a cause of Usher syndrome type 3B (USH3B) [MIM:614504]. USH3B is a syndrome characterized by progressive vision and hearing loss during early childhood. Some patients have the so-called 'Charles Bonnet syndrome,' involving decreased visual acuity and vivid visual hallucinations. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa with sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH3 is characterized by postlingual, progressive hearing loss, variable vestibular dysfunction, and onset of retinitis pigmentosa symptoms, including nyctalopia, constriction of the visual fields, and loss of central visual acuity, usually by the second decade of life.
  • Sequence similaritiesBelongs to the class-II aminoacyl-tRNA synthetase family.
    Contains 1 WHEP-TRS domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • cytoplasmic antibody
    • EC antibody
    • FLJ20491 antibody
    • HARS antibody
    • HisRS antibody
    • Histidine tRNA ligase, cytoplasmic antibody
    • histidine translase antibody
    • Histidine tRNA ligase antibody
    • Histidine--tRNA ligase antibody
    • Histidyl tRNA synthetase antibody
    • Histidyl-tRNA synthetase antibody
    • HRS antibody
    • Human histidyl tRNA synthetase homolog (HO3) mRNA complete cds antibody
    • SYHC_HUMAN antibody
    • USH3B antibody
    see all

Anti-HARS antibody images

  • ab65014 at 1/500 - 1/1000 dilution staining HARS in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • All lanes : Anti-HARS antibody (ab65014) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa cell extract with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Additional bands at : 20 kDa,37 kDa,40 kDa. We are unsure as to the identity of these extra bands.

References for Anti-HARS antibody (ab65014)

This product has been referenced in:
  • Sciorati C  et al. Required role of apoptotic myogenic precursors and toll-like receptor stimulation for the establishment of autoimmune myositis in experimental murine models. Arthritis Rheumatol 67:809-22 (2015). ICC/IF ; Mouse . Read more (PubMed: 25504878) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (C2C12)
Permeabilization Yes - triton 0.1%
Specification C2C12
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Paraformaldehyde

antonella Monno

Verified customer

Submitted Apr 28 2015